| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Septin7 recombinant protein (amino acids M1-K348) was used as the immunogen for the Zebrafish Septin7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Septin7 Antibody / Septin7a / Septin7b is a anti-SEPTIN7 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: SEPTIN7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Septin7 is an ortholog of the human SEPTIN7 protein, with strong sequence and functional conservation across species. In zebrafish, Septin7 is encoded by 2 isoforms, Septin7a and Septin7b. Both isoforms share similar functions but may exhibit differences in expression patterns during development or in specific tissues. The presence of Septin7a and Septin7b in zebrafish provides a unique opportunity to study how septin isoforms cooperate or diverge in their roles within the cytoskeleton and signaling pathways.
Septin7 is particularly important during cellular processes that require cytoskeletal remodeling, such as neurite outgrowth, epithelial polarity, and germ cell development. Disruption of Septin7 function can lead to defects in cytokinesis, altered vesicle trafficking, and impaired cell signaling.
Antibodies against zebrafish Septin7 protein are useful for studying the organization of septin filaments and their interactions with actin and microtubule networks. These antibodies are valuable in applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA. The conservation of Septin7 between zebrafish and human systems also makes zebrafish a powerful model for exploring the fundamental roles of SEPTIN7 in cell division, morphogenesis, and disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
