| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Smarca4a recombinant protein (amino acids Q573-E775) was used as the immunogen for the Zebrafish Smarca4a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Smarca4a Antibody / Smarca4 is a anti-SMARCA4A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear.
Key elements and design rationale
- Target: SMARCA4A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P, IF
Biological background
Smarca4a in zebrafish is an ortholog of the human SMARCA4 protein also widely known as Brg1. The zebrafish protein shows strong sequence and functional conservation with the human enzyme, supporting the use of zebrafish as a model to study conserved mechanisms of chromatin remodeling and their roles in development and disease. Zebrafish also carries a related paralog named Smarca4b, which allows comparative analyses of partially overlapping and partially specialized functions within the genome duplication context of teleosts.
Smarca4a is broadly expressed during zebrafish development, with higher levels in rapidly proliferating and differentiating tissues where large scale transcriptional reprogramming is required. Loss of Smarca4a function disrupts body patterning, organogenesis, and cell fate specification, underscoring its central role in controlling chromatin structure and transcription.
Antibodies directed against zebrafish Smarca4a are valuable reagents for studying epigenetic regulation, transcriptional control, and developmental pathways. Typical applications include western blot to assess protein abundance, immunohistochemistry and immunofluorescence to map spatial expression in embryos and tissues, chromatin immunoprecipitation to define genomic binding sites, and immunoprecipitation based assays to characterize Swi Snf complex composition.
Because zebrafish Smarca4a is an ortholog of human SMARCA4, findings obtained with these antibodies can inform research on human developmental disorders, cancer, and other diseases linked to defects in chromatin remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
