| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Sod2 recombinant protein (amino acids M1-K224) was used as the immunogen for the Zebrafish Sod2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Sod2 Antibody / Superoxide dismutase 2 is a anti-SOD2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: SOD2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Sod2 is an ortholog of the human SOD2 protein, with strong sequence and functional conservation. As in humans, Sod2 in zebrafish plays a vital role in protecting highly metabolic tissues such as the brain, heart, and muscles from oxidative damage. Mutations or reduced activity of Sod2 can lead to mitochondrial dysfunction and oxidative stress, contributing to developmental abnormalities and degenerative diseases.
Currently, there are no confirmed isoforms of zebrafish Sod2. It is broadly expressed across tissues but is particularly abundant in cells with high energy demands. Zebrafish models deficient in Sod2 have been used to investigate the consequences of oxidative imbalance and to explore therapeutic approaches aimed at reducing mitochondrial stress.
Antibodies targeting zebrafish Sod2 protein are important tools for studying mitochondrial antioxidant defense systems, oxidative signaling pathways, and stress responses. These antibodies can be utilized in western blot, immunohistochemistry, immunofluorescence, and ELISA to monitor Sod2 levels and localization in zebrafish tissues.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
