| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Sox32 recombinant protein (amino acids M1-K307) was used as the immunogen for the Zebrafish Sox32 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Sox32 Antibody / Casanova is a anti-SOX32 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: SOX32
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Sox32 is considered a functional counterpart to members of the human SOX family, although there is no single direct human ortholog due to evolutionary divergence. The conserved role of Sox family proteins in regulating developmental gene expression makes Sox32 a critical target for studying early embryogenesis and cell lineage determination.
The use of a Zebrafish Sox32 antibody enables researchers to detect and study the expression patterns of Sox32 during early development. Such antibodies are essential tools for applications including western blot, immunohistochemistry, and immunofluorescence to visualize Sox32 in specific tissues or cell types. By applying a Zebrafish Sox32 antibody, scientists can gain deeper insights into the transcriptional networks that control endoderm differentiation and organogenesis.
There are no known isoforms of zebrafish Sox32, and its expression is tightly regulated during the early stages of development. Reagents such as a Zebrafish Sox32 antibody are also useful in exploring how perturbations in Sox32 function can lead to defects in endoderm specification or other developmental abnormalities.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
