| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Tgfb1 recombinant protein (amino acids A266-S377) was used as the immunogen for the Zebrafish Tgfb1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Tgfb1 Antibody / Tgfb1a / Tgf beta is a anti-TGFB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: TGFB1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Zebrafish TGF-beta proteins belong to the TGF-beta superfamily, which also includes activins, nodal, and bone morphogenetic proteins (BMPs). These ligands signal through serine/threonine kinase receptors, leading to the phosphorylation of receptor-regulated SMADs (R-Smads), which then form complexes with co-Smad (Smad4) and translocate to the nucleus to regulate gene expression.
TGF-beta signaling in zebrafish is critical during embryogenesis and tissue morphogenesis. It plays essential roles in mesoderm formation, left-right axis specification, cardiovascular development, and immune system modulation. Specific zebrafish isoforms, such as tgfb1a, tgfb1b, and tgfb3, exhibit distinct expression patterns and developmental functions. For example, tgfb1b is involved in hematopoiesis and immune regulation, while tgfb3 contributes to craniofacial development and epithelial integrity.
Due to the genetic tractability and optical transparency of zebrafish embryos, this model system provides unique advantages for in vivo analysis of TGF-β pathway dynamics. Zebrafish TGF-beta proteins serve as valuable tools for studying developmental biology, regeneration, fibrosis, and disease mechanisms, including cancer and immune dysfunction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
