| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Xpo1 recombinant protein (amino acids H988-L1054) was used as the immunogen for the Zebrafish Xpo1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Xpo1 Antibody / Xpo1a / Xpo1b / Exportin 1 is a anti-XPO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: XPO1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Xpo1 is an ortholog of the human XPO1 protein, and the two share significant sequence and functional conservation. Similar to human Xpo1, zebrafish Xpo1 is involved in the transport of a wide variety of cargo molecules, including ribosomal subunits and RNA species such as mRNA, tRNA, and snRNA. This makes Xpo1 essential for cellular homeostasis and the proper functioning of many cellular systems, including gene expression, protein synthesis, and stress responses.
Zebrafish Xpo1 exists in multiple isoforms, which may have specialized roles in different tissues or developmental stages. The primary isoform is responsible for most of the transport activity, while other isoforms may be involved in specific cargo recognition or subcellular localization. The diversity of Xpo1 isoforms allows for fine-tuned regulation of nuclear export and contributes to the adaptability of the cellular transport system in zebrafish.
The use of a Zebrafish Xpo1 antibody allows researchers to study the expression, localization, and function of Xpo1 during various stages of zebrafish development. Zebrafish Xpo1 antibody reagents are essential tools for applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA, enabling the detection and analysis of Xpo1 isoforms and their involvement in nuclear export pathways. A Zebrafish Xpo1 antibody can help identify how perturbations in Xpo1 function affect cellular processes like mRNA transport, protein synthesis, and cell signaling.
By using a high-quality Zebrafish Xpo1 antibody, scientists can gain insights into the regulation of nuclear-cytoplasmic transport, a crucial aspect of cellular function that is conserved across species.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
