Validated Cancer Antibodies

Application-validated cancer-research antibodies, each validated for two or more applications spanning Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications validated for your target. Save 15% with code ONCO15.

4373Products
4373 products
Image ProductProduct Type ApplicationPrice Status ACTION
Exposure time: 120s
Target: EGFR
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Target: EGFR
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
From $119.00
Regular price From $119.00
In Stock at Manufacturer
EGFR Polyclonal Antibody – Figure 1 of 4
Polyclonal Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $73.00
Regular price From $73.00
In Stock at Manufacturer
EGFR-Specific antibody – Figure 1 of 5
Target: EGFR
Primary Antibody ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
$312.00
Regular price $312.00
In Stock at Manufacturer
Immunofluorescent analysis using the EIF3A antibody (green) at 1:50 dilution.
Target: EIF3A
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
EIF4A3 antibody – Figure 1 of 4
Target: EIF4A3
Primary Antibody ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
$312.00
Regular price $312.00
In Stock at Manufacturer
Western blot analysis of extracts from 293 and HepG2 cells using 4E-BP1(Ab-45) Antibody.
Target: EIF4EBP1
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
$297.00
Regular price $297.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human ovarian cancer tissue with EML4 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Target: EML4
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$449.00
Regular price $449.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A431 whole cell lysate
All lanes: ENO1 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48, 37 kDa
Observed band size: 48 kDa
Target: ENO1
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
From $166.00
Regular price From $166.00
In Stock at Manufacturer
Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human U-251, 4) mouse NIH 3T3 and 5) mouse brain lysate with ENO1 antibody. Predicted molecular weight ~47 kDa.
Target: ENO1
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain tissue, Mouse Brain tissue, Rabbit Skeletal Muscle tissue, Rat Kidney tissue, Rabbit Kidney tissue 
All lanes ENO1 antibody at 1:10000
Secondary
Goat polyclonal to mouse IgG at 0.261ug/ml
Predicted band size: 47 KDa
Observed band size: 47 KDa
Exposure time: 1min
Target: ENO1
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: HeLa whole cell lysate(20µg) , U-251MG whole cell lysate(20µg) , A549 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg) , K562 whole cell lysate(20µg) , NIH/3T3 whole cell lysate(20µg) , HEK293 whole cell lysate(20µg) , Jurkat whole cell lysate(20µg) , HepG2 whole cell lysate(20µg) 
All lanes: ENO1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa
Exposure time:5s
Target: ENO1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human A431 cells with Enolase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: ENOLASE 1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
$449.00
Regular price $449.00
In Stock at Manufacturer
EP-CAM Monoclonal Antibody – Figure 1 of 2
Target: EPCAM
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $180.00
Regular price From $180.00
In Stock at Manufacturer
Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EGP40/1373, green) and Reddot nuclear stain (red).
Target: EpCAM
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunohistochemistry
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE mouse colon tissue with Epcam antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Target: Epcam
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of human rectal cancer tissue with EpCAM antibody (red) and DAPI (blue). HIER: steam sections in pH6 citrate buffer for 20 min.
Target: EpCAM
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$449.00
Regular price $449.00
In Stock at Manufacturer
Right: Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM antibody (EGP40/826) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). Left: Negative control. DAPI was used to stain the cell nuclei (blue).
Target: EPCAM
Antibodies, Primary Antibodies Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
From $259.00
Regular price From $259.00
In Stock at Manufacturer
IHC testing of FFPE human ovarian carcinoma and EpCAM antibody (clone EGP40/1384). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
Target: EPCAM
Antibodies, Primary Antibodies ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
From $259.00
Regular price From $259.00
In Stock at Manufacturer
IHC testing of FFPE human fallopian tube with EpCAM antibody (clone EPM17-4). Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Target: EpCAM
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunohistochemistry
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot testing of human lung lysate with EpCAM antibody cocktail (clone PAN-EpCAM).
Target: EPCAM
Antibodies, Primary Antibodies Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human PC-3 cells with Eph receptor A3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: Eph receptor A3
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$449.00
Regular price $449.00
In Stock at Manufacturer
IHC testing of FFPE human endometrium with ER antibody (clone ER506).
Target: ER
Antibodies, Primary Antibodies Flow Cytometry, Western Blot, Immunohistochemistry, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
IHC testing of FFPE human brain tissue with ERAS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Target: ERAS
Antibodies, Primary Antibodies Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western blot analysis of extracts from SKOV3 and MDA cells using HER2(Ab-1221/1222) Antibody.
Target: ERBB2
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
$297.00
Regular price $297.00
In Stock at Manufacturer
Western blot analysis of extracts from MCF and SKOV3 cells using HER2(Ab-1248) Antibody.
Target: ERBB2
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
$297.00
Regular price $297.00
In Stock at Manufacturer
ERBB3 Antibody – Figure 1 of 1
Target: ERBB3
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $119.00
Regular price From $119.00
In Stock at Manufacturer
ERBB3 Antibody – Figure 1 of 1
Target: ERBB3
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $119.00
Regular price From $119.00
In Stock at Manufacturer
Western blot testing of ERK1 antibody and Lane 1: rat spleen; 2: rat thymus; 3: rat skeletal muscle; 4: rat kidney; 5: HeLa; 6: Jurkat; 7: Raji; 8: COLO320. Predicted molecular weight: ~44 kDa.
Target: ERK1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Rabbit Anti-Erk1/2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-120)
Target: Erk1/2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $288.00
Regular price From $288.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human U-2 OS cells with ERp5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: ERP5
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing of human 1) PANC-1, 2) 22RV1, 3) COLO-320, 4) SW620 and 5) mouse SP20 lysate with ESRRG antibody at 0.5ug/ml. Predicted molecular weight ~51 kDa.
Target: ESRRG
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$449.00
Regular price $449.00
In Stock at Manufacturer
Estrogen Receptor antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma.
Target: ESR1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
IHC testing of human breast cancer stained with Estrogen Receptor beta antibody (ERb455).
Target: ESTROGEN
Antibodies, Primary Antibodies Flow Cytometry, Immunohistochemistry, Western Blot, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Estrogen Receptor beta antibody (ESR2/686).
Target: ESTROGEN
Antibodies, Primary Antibodies Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western Blot analysis of various cells using Phospho-Ets-1 (T38) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $192.40
Regular price From $192.40
In Stock at Manufacturer

About Validated Cancer Antibodies

Every antibody here is validated for multiple cancer-research applications — two or more of Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications and images validated for your specific target. Use code ONCO15 for 15% off cancer antibodies.

How validated is “validated”? Choosing a cancer antibody

Two antibodies to the same target are not interchangeable. The deciding factor is the evidence behind the listing — in which applications, species, and with what images. This collection sets the bar at multi-application validation: every antibody is validated in two or more of WB, IHC and IF.

Decision dimension Multi-application validated (this collection) Single-application Catalog / RUO-only
Validation evidenceTwo or more of WB / IHC / IF, with datasheet dataOne application (often WB only)Specification sheet; little or no application data
Applications confirmed2+ (across lysate, tissue and/or cells)10–1, frequently predicted
Reproducibility riskLower — cross-application concordanceModerate — format-dependentHigher — unverified
Best forPublication-grade tumor work across methodsA single, well-defined assayEarly screening on a budget
Watch-outsConfirm the validated applications, species and clone for your modelMay not transfer to IHC/IFPlan to validate in-house first

How to read this: if your project spans lysate, tissue and cells — or is headed for publication — start here, where every antibody is validated in two or more of these applications. Confirm on the datasheet which applications are validated for your target. If you only need one assay, the broader Cancer Antibodies catalog has more single-application options; for non-cancer targets browse all Antibodies. Validation is per-clone and per-lot — always check the datasheet image for your specific application and species.

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