Validated Cancer Antibodies

Application-validated cancer-research antibodies, each validated for two or more applications spanning Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications validated for your target. Save 15% with code ONCO15.

2679Products
2679 products
Image ProductProduct Type ApplicationPrice Status ACTION
Western blot testing of 1) rat thymus, 2) rat spleen, 3) human MCF7, 4) HeLa, 5) Jurkat, and 6) mouse NIH3T3 lysate with CDK1 antibody. Predicted/observed molecular weight: ~33 kDa.
Target: CDK1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Flow Cytometry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human A431 cells with Pokemon antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: ZBTB7A
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
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Western blot testing of 1) rat liver, 2) mouse liver and 3) human SMMC lysate with ABCB11 antibody. Expected/observed molecular weight ~146 kDa.
Target: ABCB11
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
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Western blot testing of 1) mouse skeletal muscle and 2) human HeLa lysate with PPP1R12A antibody. Expected molecular weight: 110~130 kDa, observed here at ~150 kDa.
Target: PPP1R12A
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Flow Cytometry Immunofluorescence
$449.00
Regular price $449.00
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Western blot testing of RUNX3 antibody and human lysates 1. A431 and 2. U-2 0S. Predicted molecular weight: ~44 kDa, isoforms can be observed at 42-48 kDa.
Target: RUNX3
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Flow Cytometry Immunofluorescence
$449.00
Regular price $449.00
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IHC-P: Vimentin antibody testing of human breast cancer tissue. Required HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: VIM
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
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Immunofluorescent staining of FFPE human A431 cells with NMI antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: NMI
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
IHC staining of FFPE human colon cancer tissue with PIN1 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: PIN1
Antibodies, Primary Antibodies Western Blot Flow Cytometry Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human tonsil with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: CD44
Antibodies, Primary Antibodies Western Blot Immunofluorescence Immunohistochemistry
$449.00
Regular price $449.00
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Western blot testing of PRDX3 antibody and Lane 1: rat brain; 2: mouse brain; 3: rat skeletal muscle; 4: mouse skeletal muscle; 5: human U-2 0S; 6: HeLa; 7: SMMC-7721; 8: RH35; 9: A549 lysate. Expected molecular weight: ~28 kDa.
Target: PRDX3
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing of human 1) U-87 MG, 2) HeLa, 3) MDA-MB-453, 4) PC-3, 5) A431, 6) Caco-2 and 7) HepG2 cell lysate with LYRIC antibody. Expected molecular weight: 70-80 kDa.
Target: LYRIC
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human intestinal cancer tissue with Nucleophosmin antibody. HIER: steam section in pH8 EDTA for 20 min.
Target: NPM1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
IHC-P: PARP antibody testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
Target: PARP
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
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IHC staining of FFPE human breast cancer tissue with NEDD9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: NEDD9
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing of human 1) HEK293, 2) HEK293 (different lot), 3) HeLa, 4) COLO-320, 5) T-47D and 6) A549 cell lysate with MSH2 antibody. Expected molecular weight: ~105 kDa.
Target: MSH2
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human MCF7 cells with MGMT antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: MGMT
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing of 1) rat brain, 2) rat kidney, 3) rat lung, 4) rat liver, 5) mouse brain, 6) mouse kidney, 7) mouse lung, 8) mouse liver and 9) mouse NIH3T3 lysate. Predicted/observed molecular weight ~83 kDa.
Target: MAD1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing of 1) rat brain, 2) rat heart, 3) rat thymus, 4) rat RH35, 5) human HeLa, 6) human COLO-320, 7) human HepG2, 8) mouse HEPA1-6 and 9) human MCF7 cell lysate with Plakoglobin antibody. Expected molecular weight: ~86 kDa.
Target: JUP
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human glioma tissue with Calveolin-1 antibody (red) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Target: CAV1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
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Immunofluorescent staining of FFPE rectal cancer with Caveolin-2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Target: CAV2
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Flow Cytometry Immunofluorescence
$449.00
Regular price $449.00
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Western blot testing of 1) lysate from untreated human Jurkat cells, 2) lysate from Jurkat cells treated with 1 uM staurosporine, 3) lysate from Jurkat cells treated with 2 uM staurosporine and 4) lysate from untreated human HepG2 cell lysate with Caspase-3 antibody.
Target: CASP3
Antibodies, Primary Antibodies Western Blot Immunofluorescence Immunohistochemistry
$449.00
Regular price $449.00
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Immunofluorescent staining of FFPE human SH-SY5Y cells with GSTP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: GSTP1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human U-2 OS cells with FHIT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: FHIT
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
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Immunofluorescent staining of FFPE human prostate hyperplasia tissue with p63 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Target: p63
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
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Western blot testing of p27KIP1 antibody and Lane 1: rat thymus; 2: rat brain lysate. Expected/observed molecular weight ~27 kDa.
Target: p27KIP1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
IHC staining of FFPE human glioma with CD34 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: CD34
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing of Hsp90 alpha antibody and Lane 1: rat liver; 2: HeLa; 3: MCF-7; 4: Raji lysate. Expected/observed molecular weight: 86-90 kDa.
Target: HSP90AA1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human HeLa cells with Beclin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: Beclin 1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Flow Cytometry Immunofluorescence Immunoprecipitation
$449.00
Regular price $449.00
In Stock at Manufacturer
IHC staining of FFPE human intestinal cancer with ITGB4 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Target: ITGB4
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human A431 cells with p53 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: p53
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing of 1) human HepG2, 2) human PC-3, 3) monkey COS-7, 4) rat liver, 5) mouse liver and 6) mouse kidney tissue lysate with HYAL1 antibody. Expected molecular weight: 48-60 kDa.
Target: HYAL1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing of rat 1) heart, 2) kidney, 3) lung and human 4) A549, 5) A431 and 6) COLO320 lysate with WISP1 antibody. Expected molecular weight: 40-55 kDa depending on degree of glycosylation.
Target: WISP1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human A431 cells with Annexin VII antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: VII
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human A431 cells with p63 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: p63
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
$449.00
Regular price $449.00
In Stock at Manufacturer
Western blot testing with VEGFR2 antibody and SMMC-7721 lysate. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).
Target: VEGFR2
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer
Immunofluorescent staining of FFPE human U-2 OS cells with NRG1 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Target: NRG1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence
$449.00
Regular price $449.00
In Stock at Manufacturer

About Validated Cancer Antibodies

Every antibody here is validated for multiple cancer-research applications — two or more of Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications and images validated for your specific target. Use code ONCO15 for 15% off cancer antibodies.

How validated is “validated”? Choosing a cancer antibody

Two antibodies to the same target are not interchangeable. The deciding factor is the evidence behind the listing — in which applications, species, and with what images. This collection sets the bar at multi-application validation: every antibody is validated in two or more of WB, IHC and IF.

Decision dimension Multi-application validated (this collection) Single-application Catalog / RUO-only
Validation evidenceTwo or more of WB / IHC / IF, with datasheet dataOne application (often WB only)Specification sheet; little or no application data
Applications confirmed2+ (across lysate, tissue and/or cells)10–1, frequently predicted
Reproducibility riskLower — cross-application concordanceModerate — format-dependentHigher — unverified
Best forPublication-grade tumor work across methodsA single, well-defined assayEarly screening on a budget
Watch-outsConfirm the validated applications, species and clone for your modelMay not transfer to IHC/IFPlan to validate in-house first

How to read this: if your project spans lysate, tissue and cells — or is headed for publication — start here, where every antibody is validated in two or more of these applications. Confirm on the datasheet which applications are validated for your target. If you only need one assay, the broader Cancer Antibodies catalog has more single-application options; for non-cancer targets browse all Antibodies. Validation is per-clone and per-lot — always check the datasheet image for your specific application and species.

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