rAAV-hSyn-SV40 NLS-Cre

BC-0159

hSyn

SV40 NLS-Cre

1050

AAV1; AAV5; AAV8; AAV9; AAV.PHP.eB; AAV2; AAV6; AAV.rh10; AAV.DJ; AAV.DJ8; AAV.PHP.B; AAV.PHP.S; AAV2/RETRO; AAV.anc80

AAV

This AAV expresses SV40 NLS-Cre driven by a neuron hSyn promoter.
hSyn is a Human synapsin 1 gene promoter.
Cre is a recombinase from the P1 bacteriophage. This enzyme carries out site-specific recombination between two DNA recognition sites (LoxP sites) through a topoisomerase I-like mechanism. The Cre recombinase here has been codon-optimized leading to a higher level of Cre protein [removed]iCre). The 34base pair (bp) loxP recognition site, ATAACTTCGTATA ATGTATGC TATACGAAGTTAT, consists of two 13 bp palindromic sequence which flank an 8 bp spacer region. The products of Cre-mediated recombination at loxP sites are dependent upon the location and relative orientation of the loxP sites. For example, two separate DNA species both containing loxP sites can undergo fusion as the result of Cre-mediated recombination. DNA sequences found between two loxP sites on the same DNA molecule are said to be floxed. In this case, the products of Cre-mediated recombination depend upon the orientation of the loxP sites: DNA found between two loxP sites oriented in the same direction will be excised as a circular loop of DNA, whereas DNA between two loxP sites that are oriented oppositely will be inverted. Besides LoxP sites, other Lox sites have been designed and tested, such as Lox272 and LoxN etc. These Lox elements have been widely used for genetic studies.
SV40 NLS is nuclear localization signal (NLS) derived from SV40 T antigen.

2×10¹² vg/ml-5×10¹² vg/ml
If you need a higher titer, please email us at [email protected]

Iodixanol gradient ultracentrifugation

Store at -80℃. Thaw just before use and keep on ice.