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| Assay Time | |
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| Product Type | |
| Sample Type(s) | Cell culture |
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Overview
For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis. The assay uses OD500nm for signal readout. Compatible sample input includes Cell culture. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD500nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Cell culture, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 20 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Safe. Non-radioactive assay (cf. chromium release assay).
Additional feature notes highlight Fast. The whole procedure takes 20 min; Robust and amenable to HTS. Single reagent, “mix-incubate-measure” type assay. High-throughput assay in 96-well plates allows the simultaneous processing of tens of thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of ldh cytotoxicity within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme that upon membrane damage is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage. The colorimetric LDH release assay is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in the cell culture supernatant. The assay is based on the reduction of a tetrazolium salt to a formazan dye.
Detection method
Colorimetric (OD 500 nm).
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify cell viability or cytotoxicity in cultured cells by OD500 nm readout.
- Compare dose-response effects of compounds across treated cell-culture wells.
- Monitor cytotoxicity time-courses after toxin, detergent, or drug exposure in culture.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does this assay kit work for a particular cell line?
Yes, this kit works for all cell lines of any species.
Why is there no standard for this assay?
This assay calculates the percentage of cell death. Therefore it uses the Total Lysis well and the No Treatment Control well (instead of a standard) to calculate the percentage of cell death in your samples.
How long do I need to incubate my test compounds with my cells?
This depends entirely on your test compound and your experiment. The incubation can be as short as 5 minutes to as long as one day. The Triton-X 100 total lysis reagent acts immediately to lyse the cells and can be read immediately if so desired.
What is the best way to store the reagent?
If you will be using the assay constantly every day, then store in the refridgerator. For long term storage, freeze the reagent at -20°C.
When does the assay expire?
The shelf life of the assay is at least one year after receipt at -20°C.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Exploring Galleria mellonella larval model to evaluate antibacterial efficacy of Cecropin A (1-7)- Melittin against multi-drug resistant enteroaggregative Escherichia coli
Vergis, J et al (2021). Exploring Galleria mellonella larval model to evaluate antibacterial efficacy of Cecropin A (1-7)- Melittin against multi-drug resistant enteroaggregative Escherichia coli. Pathogens and Disease, ftab010. Assay: LDH Cytotoxicity in galleria mellonella.
Retrospective analysis of the efficacy of adjuvant CIK cell therapy in epithelial ovarian cancer patients who received postoperative chemotherapy
Zhou, et al (2019). Retrospective analysis of the efficacy of adjuvant CIK cell therapy in epithelial ovarian cancer patients who received postoperative chemotherapy. OncoImmunology, 8(2), e1528411. Assay: LDH Cytotoxicity in human cells.
Heme-induced acute lung injury by inflammasome activation in vascular endothelium (Doctoral dissertation, University of Pittsburgh)
Flage, B. (2018). Heme-induced acute lung injury by inflammasome activation in vascular endothelium (Doctoral dissertation, University of Pittsburgh). Assay: Cytotoxicity in human tissues.
