| Field | Specification |
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| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, urine, saliva, food and beverage etc |
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Overview
For quantitative determination of total antioxidant capacity (TAC) and evaluation of drug effects on TAC metabolism. The assay uses OD570nm for signal readout. Compatible sample input includes Serum, plasma, urine, saliva, food and beverage etc. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD570nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, saliva, food and beverage etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 1.5 µM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use 20 µL sample. Linear detection range from 1.5 to 1000 µM Trolox equivalents.
Additional feature notes highlight Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of antioxidant within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
An ANTIOXIDANT is a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline. Simple, direct and high-throughput assays for total antioxidant capacity (TAC) find wide applications in research, food industry and drug discovery. BioAssay Systems improved assay measures total antioxidant capacity in which Cu2+is reduced by antioxidant to Cu+. The resulting Cu+specifically forms a colored complex with a dye reagent. The color intensity at 570nm is proportional to TAC in the sample.
Detection method
Colorimetric (OD 570 nm).
Detection limit and analytical sensitivity
Reported detection limit: 1.5 µM.
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify antioxidant in serum, plasma, urine by OD570 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, urine handling.
- Monitor time-course or pre/post changes in serum, plasma, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What substances are known to interfere with the antioxidant assay?
Detergents, such as TWEEN-20, TRITON X-100, NP-40, should not be present at any concentration in the test sample. Reducing materials (DTT, 2-mercaptoethanol) and metal chelators (e.g. EDTA) may interfere with the assay.
How to determine TAC in tissue samples?
Homogenize tissue samples on ice in cold 1xPBS (~100 mg of tissue/0.5 ml of buffer) and then centrifuge at 12,000g for 15 minutes at 4°C. Remove the supernatant and keep it on ice. For long term storage, store in working aliquots at –80°C.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Evaluation of electrolyte concentration and pro-inflammatory and oxidative status in dogs with advanced chronic kidney disease under dietary treatment
Halfen, DP et al. (2020). Evaluation of electrolyte concentration and pro-inflammatory and oxidative status in dogs with advanced chronic kidney disease under dietary treatment. Toxins, 12(1), 3. Assay: Antioxidant in canine serum.
Does vitamin e supplementation enhance growth benefits of breeding helpers at no oxidative costs? Physiological and Biochemical Zoology: PBZ, 93(1): 37-48
Montoya, B et al. (2020). Does vitamin e supplementation enhance growth benefits of breeding helpers at no oxidative costs? Physiological and Biochemical Zoology: PBZ, 93(1): 37-48. Assay: Antioxidant in avian plasma.
Role of dietary gamma-aminobutyric acid in broiler chickens raised under high stocking density
Jeong, SB et al. (2020). Role of dietary gamma-aminobutyric acid in broiler chickens raised under high stocking density. Animal Nutrition, 6(3): 293-304. Assay: Antioxidant in chicken serum.
Dietary encapsulated essential oils improve production performance of coccidiosis-vaccine-challenged broiler chickens
Lee, JW et al. (2020). Dietary encapsulated essential oils improve production performance of coccidiosis-vaccine-challenged broiler chickens. Animals: An Open Access Journal from MDPI, 10(3). Assay: Antioxidant in chicken serum.
Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
Vieira da Silva, I et al. (2021). Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets. PloS One, 16(1), e0245739. Assay: Antioxidant in pig serum.
Black soldier fly larvae oil as an alternative fat source in broiler nutrition
Kim, YB et al. (2020). Black soldier fly larvae oil as an alternative fat source in broiler nutrition. Poultry Science, 99(6): 3133-3143. Assay: Antioxidant in chicken serum.
Research Note: Effects of supplementing cranberry and blueberry pomaces on meat quality and antioxidative capacity in broilers
Xu, Q et al. (2020). Research Note: Effects of supplementing cranberry and blueberry pomaces on meat quality and antioxidative capacity in broilers. Poultry Science, 100900. Assay: Antioxidant in chicken tissue.
Lipid peroxidation in the descending thoracic aorta of rats deprived of REM sleep using the inverted flowerpot technique
Nawi, A et al. (2020). Lipid peroxidation in the descending thoracic aorta of rats deprived of REM sleep using the inverted flowerpot technique. Experimental Physiology, 105(8): 1223-1231. Assay: Antioxidant in rat tissue.
Consumption of nixtamal from a new variety of hybrid blue maize ameliorates liver oxidative stress and inflammation in a high-fat diet rat model
Magana-Cerino, JM et al. (2020). Consumption of nixtamal from a new variety of hybrid blue maize ameliorates liver oxidative stress and inflammation in a high-fat diet rat model. Journal of Functional Foods, 72, 104075. Assay: Antioxidant in rat liver.
Analysis of the Total Antioxidant Capacity of Saliva in Smokers
Indrastiti, R. K., Rahmayanti, F., & Soegyanto, A. I. (2019). Analysis of the Total Antioxidant Capacity of Saliva in Smokers. Pesquisa Brasileira em Odontopediatria e Clinica Integrada, 19(1), 4897. Assay: Antioxidant in human saliva.
Oral Glutamine Supplement Reduces Subjective Fatigue ratings during Repeated Bouts of Firefighting Simulations
Moore, M., Moriarty, T. A., Connolly, G., Mermier, C., Amorim, F., Miller, K., & Zuhl, M. (2019). Oral Glutamine Supplement Reduces Subjective Fatigue ratings during Repeated Bouts of Firefighting Simulations. Safety, 5(2), 38. Assay: Antioxidant in horseshoe crab serum.
Dynamic, Not Isometric Resistance Training Improves Muscle Inflammation, Oxidative Stress and Hypertrophy in rats
Neves, R. V. P., Rosa, T. S., Souza, M. K., Oliveira, A. J. C., Gomes, G. N. S., Brixi, B. & Prestes, J. (2019). Dynamic, Not Isometric Resistance Training Improves Muscle Inflammation, Oxidative Stress and Hypertrophy in rats. Frontiers in physiology, 10. Assay: Antioxidant in rat muscle tissue.
iNOS polymorphism modulates iNOS/NO expression via impaired antioxidant and ROS content in P
Kumar, A., Singh, K. P., Bali, P., Anwar, S., Kaul, A., Singh, O. P. & Sinha, M. P. (2018). iNOS polymorphism modulates iNOS/NO expression via impaired antioxidant and ROS content in P. vivax and P. falciparum infection. Redox biology, 15, 192-206. Assay: Antioxidant in human serum.
Characterization and Cultivation of Marine Protist, Thraustochytrium striatum for High-Value Bioproducts
Xiao, R. (2018). Characterization and Cultivation of Marine Protist, Thraustochytrium striatum for High-Value Bioproducts. Assay: Antioxidant in mouse cells.
Effects of diets with whole plant-origin proteins added with different ratios of taurine: methionine on the growth, macrophage activity and antioxidant capacity of rainbow trout (Oncorhynchus mykiss) fingerlings
Hernandez, O. E. A., Hernandez, L. H. H., Miyasaka, A. S., & Lugo, M. G. (2017). Effects of diets with whole plant-origin proteins added with different ratios of taurine: methionine on the growth, macrophage activity and antioxidant capacity of rainbow trout (Oncorhynchus mykiss) fingerlings. Veterinary and Animal Science, 3, 4-9. Assay: Antioxidant in fish liver tissue.
Better management of alcohol liver disease using a ‘Microstructured Synbox’ System comprising L
Rishi, P., Arora, S., Kaur, U. J., Chopra, K., & Kaur, I. P. (2017). Better management of alcohol liver disease using a ‘Microstructured Synbox’ System comprising L. plantarum and EGCG. PloS one, 12(1), e0168459. Assay: Antioxidant in rat liver tissue.
Novel phosphate-activated macrophages prevent ectopic calcification by increasing extracellular ATP and pyrophosphate
Villa-Bellosta, R., Hamczyk, M. R., & Andres, V. (2017). Novel phosphate-activated macrophages prevent ectopic calcification by increasing extracellular ATP and pyrophosphate. PloS one, 12(3), e0174998. Assay: Antioxidant in mice marrow.
Comparison of serum Total Antioxidant Capacity between metabolic syndrome patients and non-metabolic syndrome people with normal or high BMI
Hasaninasab, A., Esteghamati, A., Azam, K., Yosaee, S., Habibi, N., & Djafarian, K. (2016). Comparison of serum Total Antioxidant Capacity between metabolic syndrome patients and non-metabolic syndrome people with normal or high BMI. Journal of Nutritional Sciences and Dietetics. Assay: Antioxidant in human serum.
Association of level of cognition with sympathovagal imbalance and cardiovascular risks in prehypertension
Subathra, T. A., Pal, G. K., Dhanalakshmi, Y., Nanda, N., & Swaminathan, R. P. (2016). Association of level of cognition with sympathovagal imbalance and cardiovascular risks in prehypertension. International Journal of Clinical and Experimental Physiology, 3(4), 197-203. Assay: Antioxidant in human blood.
A 16-week study to compare the effect of vildagliptin versus gliclazide on postprandial lipoprotein concentrations and oxidative stress in patients with type 2 diabetes inadequately controlled with metformin monotherapy
Hissa, MR et al (2015). A 16-week study to compare the effect of vildagliptin versus gliclazide on postprandial lipoprotein concentrations and oxidative stress in patients with type 2 diabetes inadequately controlled with metformin monotherapy. Diabetol Metab Syndr. 11;7:62. Assay: Antioxidant in human serum.
Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration
Madrigal-Bujaidar, E et al (2015). Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration. World J Gastroenterology. 21(25): 7718-7729. Assay: Antioxidant in rat liver cells.
Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration
Madrigal-Santillan, E et al (2015). Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration. World J Gastroenterology. 21(25): 7718-7729. Assay: Antioxidant in wistar rat liver cells.
Oxidant Hepatic & /or Haem
Hegazi, RM and Kamel HFM (2014). Oxidant Hepatic & /or Haem. Injury on Fuel-StationWorkers Exposed to Benzene Vapor, Possible Protectionof Antioxidants. American Journal of Medicine and Medical Sciences 4(2): 35-46. Assay: Antioxidant in human serum.
Effects of chronic nicotine administration on body weight, food intake and nitric oxide concentration in female and male rats
Ijomone, OM, Olaibi, OK, Nwoha, PU, (2014). Effects of chronic nicotine administration on body weight, food intake and nitric oxide concentration in female and male rats. Pathophysiology. 21:185-190. Assay: Antioxidant in wistar rat serum.
Histological and Histomorphometric studies of ethanol-injured pylorus and duodenum of Wistar rats pre-treated with Moringa oliefera extract
Olaibi, OK et al (2014). Histological and Histomorphometric studies of ethanol-injured pylorus and duodenum of Wistar rats pre-treated with Moringa oliefera extract. Al Am een J Med Sci. 7(2):104-111. Assay: Antioxidant in human duodenum tissue.
Chemical and Phytochemical Properties and Antioxidant Activities of Three Pomegranate Cultivars Grown in South Africa
Fawole, O., et al. (2011). Chemical and Phytochemical Properties and Antioxidant Activities of Three Pomegranate Cultivars Grown in South Africa. Food and Bioprocess Technology:1-7. Assay: Antioxidant in plant pomegranate juice.
Serum total antioxidant capacity in patients with multiple sclerosis
Hadzovi -Dzuvo, A et al (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum.
Serum total antioxidant capacity in patients with multiple sclerosis
Hadzovi -Dzuvo, A., et al. (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum.
Serum total antioxidant capacity in patients with multiple sclerosis
Hadzovic-Dzuvo, A et al (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum.
The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma
Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Total Oxidant in human blood.
