| Field | Specification |
|---|---|
| Species |
The ARPE-19 cell line, derived from the retinal pigment epithelium (RPE) of a 19-year-old male, has functional characteristics akin to native RPE cells, making it a pivotal epithelial cell model in ophthalmic research. These cells are utilized in studies related to the vertebrate retina and retinal pigment epithelium physiology. When cultured in 3D cell culture systems or as a cell monolayer on laminin-coated filters with low serum media, ARPE-19 cells achieve morphological polarization and form tight junctions, exhibiting transepithelial resistance akin to that observed in vivo.
ARPE-19 cells, expressing RPE-specific markers such as CRALBP and RPE-65, serve as an excellent model for understanding the pigmentation processes of the retinal pigment epithelium, including melanin synthesis and melanosome content.
The application of ARPE-19 human cells extends to ocular pharmacokinetics and permeability studies, providing insights into ocular chemotherapy efficacy and retinal barriers considerations. Their use in examining the interactions between pharmacokinetics and melanin content offers valuable data on drug binding and uptake. RPE-19 cells contribute to our understanding of retinal explants and the epithelium's role in eye development, given their expression of networks involved in early eye formation and muscle contraction.
In summary, the ARPE-19 cell line serves as a critical model in ophthalmic research, offering insights into the physiology of the retina, pigmentation processes, and the efficacy of ocular treatments.
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- Protein expression: Rpe-Specific Markers Cralbp And Rpe-65
- Antigen expression: RPE-specific markers CRALBP and RPE-65
- Tumorigenic: Yes
- cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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