Denarase^® ELISA Kit — The One-for-all Serratia marcescens Endonuclease Detection Kit

About the Denarase^® ELISA Kit

The Denarase^® ELISA Kit is a sandwich ELISA for quantitative in vitro measurement of residual Serratia marcescens endonuclease in process-related samples. It detects Denarase^®, Denarase^® High Salt, and Benzonase^® — providing a unified assay solution for monitoring any S. marcescens endonuclease across your downstream bioprocessing workflow.

Intended for research and manufacturing use (process development and quality control). Not for diagnostic procedures.

Detection Principle

Sandwich ELISA. Samples are incubated in 96-well plates pre-coated with a specific monoclonal capture antibody. Bound endonuclease is detected using a biotin-conjugated monoclonal detector antibody, streptavidin-HRP conjugate, and TMB colorimetric substrate. Absorbance read at OD450.

Key Performance Parameters

• Limit of Detection (LOD): 4 pg/mL
• Limit of Quantification (LOQ): 12 pg/mL
• Working Range: ~32–1,000 pg/mL
• Assay Time: ~130 minutes total incubation
• CV (OD450 Assay Control): ≤ 15%
• Capacity: 480 measurements per kit (5 × 96-well plates)
• 15–50× more sensitive than competitor ELISA kits using polyclonal antibodies
• 50% shorter assay time versus competitor kits

Kit Contents

• 5 × Microtest plates (96-well, pre-coated with monoclonal capture antibody)
• Washing Buffer (10×) — 3 × 100 mL
• Dilution Buffer (10×) — 1 × 20 mL
• Denarase^® Standard (10 µg/mL) — 1 × 0.1 mL
• Detector Antibody (100×) — biotin-conjugated monoclonal antibody, 1 × 0.75 mL
• Enzyme Conjugate (100×) — streptavidin-HRP, 1 × 0.75 mL
• Substrate Solution — TMB One, ready to use, 1 × 75 mL
• Stop Solution — 0.5 M sulfuric acid, 1 × 75 mL

Storage & Stability

• Store at 2–8 °C
• Shelf life: 18 months from production under proper storage conditions
• Shipping temperature may deviate from storage temperature without affecting product quality