| Field | Specification |
|---|---|
| Product Type | |
| Storage |
T7 RNA Polymerase is a DNA-dependent enzyme that catalyzes the synthesis of RNA in the 5' to 3' direction. It works exclusively in the presence of its T7 phage promoter sequence, ensuring high specificity for transcription. This enzyme does not recognize SP6 or T3 RNA Polymerase promoter sequences, making it ideal for applications requiring precise and controlled RNA production from T7-driven templates.
| Product Component | Quantity |
|---|---|
| T7 RNA Polymerase (50 U/μl) | 100 µl (5000 U) |
| 10X T7 RNA Polymerase Reaction Buffer | 1.0 ml |
| Specification | Value |
|---|---|
| Enzyme Type | RNA Polymerase |
| Format | Enzyme Only |
| Promoter Specificity | T7 Promoter |
| Storage Conditions | Store all components at -20°C. |
T7 RNA Polymerase specifically recognizes the consensus T7 promoter sequence (5′-TAATACGACTCACTATA-3′) and initiates transcription at the +1 adenosine. The template DNA must contain this sequence immediately upstream of the region to be transcribed.
Under optimized conditions with a linearized template (1 µg) at 37°C for 2–4 hours, T7 RNA Polymerase can yield 20–40 µg of RNA per 20 µL reaction. Circular templates produce lower yields.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- Kim Y; Nam D; Lee ES et al.. Aptamer-Based Switching System for Communication of Non-Interacting Proteins. Biosensors. 2024;14. DOI
- Abdolahzadeh A; Dolgosheina EV; Unrau PJ. RNA detection with high specificity and sensitivity using nested fluorogenic Mango NASBA. RNA (New York, N.Y.). 2019;25:1806-1813. DOI
- Lyu Z; Katolik A; Yaseen I et al.. Synthesis of long and functionally active RNAs facilitated by acetal levulinic ester chemistry. Nucleic acids research. 2026;54. DOI
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