1-EBIO

Product details

• Chemical name: 3-ethyl-1H-benzimidazol-2-one.
• Also known as: 1-Ethyl-2-benzimidazolinone
• CAS number: 10045-45-1
• Molecular formula: C9H10N2O.
• Purity: >97%
• Concentration: 5-100 μM.
• Form: Lyophilized powder.
• Solubility: 250 mM DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
• Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: KCa3.1, KCa2 channels
• Source: Synthetic
• Activity: 1-EBIO is an activator of KCa3.1 and KCa2 channel currents1.
• Alternative names: 1-Ethyl-2-benzimidazolinone

Scientific background

1-EBIO is a potent KCa2/3 channel activator that activates the channels by increasing the Ca2+ sensitivity by stabilizing the interaction between calmodulin and the channels1,2, thus shifting the activation curve of the channels to the left. 1-EBIO activates KCa3.1 with an EC50 value of around 30 μM and all three KCa2 channels with EC50 value around 300 μM2.The Ca2+-activated K+ channel, KCa3.1, plays a major role in the controlled expression of essential genes that are responsible for several physiological processes including- membrane potential, cell volume proliferation, migration and production of inflammatory mediators by participating in the regulation of calcium entry. A highly regulated and sustained increase in cytosolic Ca2+ is critical in these processes making KCa3.1 a potential target for therapy of several disease1,3.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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