1 mM Novobiocin

What it means: Bacterial DNA gyrase and topoisomerase IV — the enzyme or process this compound acts on.

Why it matters:

• Knowing the target tells you which assays the compound can serve as a control for.
• A known inhibitor in a control well shows that an assay responds as expected.
• Comparing a test compound with a reference inhibitor puts its potency in context.
• The target also helps you choose suitable concentrations for a dose-response series.
• Stating the target makes your methods easy to report and repeat.
• Reference compounds help you check day-to-day consistency of an assay.
• Knowing the mode of action helps you interpret partial or unexpected inhibition.

For this product: Novobiocin inhibits the ATPase activity of bacterial DNA gyrase (GyrB subunit) and topoisomerase IV (ParE subunit) by competing with ATP. Use it with assays for bacterial DNA gyrase and topoisomerase IV.

Tip: Run the reference compound at several concentrations to confirm that your assay responds as expected.

What it means: 1 mM — the concentration of the supplied stock.

Why it matters:

• The stock concentration decides how far you dilute the product for each reaction.
• Knowing it lets you work out how many reactions one vial supports.
• Using the same final amount in every reaction keeps results comparable.
• A stated concentration makes dilution steps easy to record and repeat.
• It also helps you compare your conditions with published methods.
• Writing down the lot and stock concentration helps when you compare runs over time.
• Over-concentrated working solutions can raise background or waste material.

For this product: The compound is supplied at 1 mM, ready to dilute into your assay buffer.

Tip: Prepare the dilution series fresh on the day of the assay.

What it means: 100 µl — how much product comes in one unit.

Why it matters:

• Pack size helps you plan how many experiments one order covers.
• It lets you set aside material for repeats and controls from the same lot.
• Knowing the amount helps you schedule reorders before you run out.
• It makes it easy to check the shipment against the order on arrival.
• Smaller sizes suit pilot work, while larger sizes cover a full study.
• The amount per unit also decides how often a vial is opened and handled.
• Ordering the right size reduces waste from material that is not used in time.

For this product: Each vial contains 100 µl of the 1 mM solution.

Tip: Plan aliquots before first use so the vial is opened as few times as possible.

What it means: Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.
• Arranging for someone to receive the package avoids delays when it arrives.
• The shipping condition is chosen to keep the product as described during transport.

For this product: Ships frozen; unpack it promptly on arrival. Please contact us to confirm storage conditions.

Tip: Open and check the package on arrival, and contact us right away if anything is damaged or missing.