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Product Overview
10 x H19 Dilution Buffer is the diluent for the H19 Dye used in DNA relaxation and supercoiling assays. It is the same dilution buffer used in the ProFoldin DNA relaxation and supercoiling assay kits and is sold separately for running additional assays. 100 ml makes 1 L of 1 x buffer, enough for about 4,000 assays at 250 µl of diluted dye per well.
Dilute the 10 x buffer 10-fold with water to make 1 x H19 Dilution Buffer, then dilute the 1500 x H19 dye in it (1 µl of dye per 1.5 ml) just before use. After the enzyme reaction, 250 µl of diluted dye is added to each 40 µl reaction and fluorescence is read at Ex 485 nm / Em 535 nm.
Product Description
| 100 ml (10 x) – H19BUF-10 | 100 ml of 10 x buffer for the H19 Dye for DNA relaxation and supercoiling assays |
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Specifications
| Concentration | 10 x |
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| Volume | 100 ml |
| Use | Diluent for the H19 Dye in DNA relaxation and supercoiling assays |
| Catalog # | H19BUF-10 |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
The ProFoldin protocol “H19 Dye for DNA Relaxation and Supercoiling Assays” is linked under Documents.
Safety & Handling
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