10 x H19 Dilution Buffer

SKU:BHT16300178
Overview
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Ten-fold concentrated buffer for diluting the H19 Dye in high-throughput DNA relaxation and supercoiling assays. Supplied as 100 ml; diluted with water to 1 x and used to prepare the working dye solution added after the reaction.
Concentration 10 x
Pack Size 100 ml
Shipping Frozen
Options selector
Catalog no. Size
H19BUF-10 100 ml (10 x)
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 100 ml (10 x) – H19BUF-10
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped frozen.
  • Upon receipt: unpack promptly.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Applications
  • Enzyme Activity
Concentration 10 x
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.
Catalog no. (Mfr.) H19BUF-10
Main SKU BHT16300178
Assay Buffers

Product Overview

10 x H19 Dilution Buffer is the diluent for the H19 Dye used in DNA relaxation and supercoiling assays. It is the same dilution buffer used in the ProFoldin DNA relaxation and supercoiling assay kits and is sold separately for running additional assays. 100 ml makes 1 L of 1 x buffer, enough for about 4,000 assays at 250 µl of diluted dye per well.

Dilute the 10 x buffer 10-fold with water to make 1 x H19 Dilution Buffer, then dilute the 1500 x H19 dye in it (1 µl of dye per 1.5 ml) just before use. After the enzyme reaction, 250 µl of diluted dye is added to each 40 µl reaction and fluorescence is read at Ex 485 nm / Em 535 nm.

Product Description

100 ml (10 x) – H19BUF-10 100 ml of 10 x buffer for the H19 Dye for DNA relaxation and supercoiling assays

Specifications

Concentration 10 x
Volume 100 ml
Use Diluent for the H19 Dye in DNA relaxation and supercoiling assays
Catalog # H19BUF-10
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.

The ProFoldin protocol “H19 Dye for DNA Relaxation and Supercoiling Assays” is linked under Documents.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.
Q.What is supplied?
A.100 ml of 10 x H19 Dilution Buffer (catalog # H19BUF-10).
Q.What is it used for?
A.It is used to dilute the H19 Dye after the enzyme reaction; it is not the enzyme reaction buffer.
Q.How is it used?
A.Dilute it 10-fold with water to make 1 x buffer, dilute the 1500 x H19 dye in the 1 x buffer (1 µl per 1.5 ml), and add 250 µl of diluted dye to each 40 µl reaction.
Q.Is a protocol available?
A.Yes. The ProFoldin protocol “H19 Dye for DNA Relaxation and Supercoiling Assays” is linked under Documents.
Q.How is it shipped and stored?
A.Shipped frozen. Please contact us to confirm storage conditions.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today