| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
100 bp DNA Ladder (100 bp Ladder DNA Marker) is composed of 12 DNA fragments with a length of 100 bp to 1,500 bp, dissolved in 1× Loading Buffer, and can be loaded directly, 5–10 µL per well, with no further preparation.
The twelve fragments are 100, 200, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,200 and 1,500 bp. The 500 bp indicator band contains 125 ng per 5 µL and every other band contains 50 ng per 5 µL. The marker is ready to use and needs no added loading buffer.
Specifications
| Fragment range | 100–1,500 bp |
|---|---|
| Number of bands | 12 |
| Band sizes | 100, 200, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,200 and 1,500 bp |
| Reference band(s) | 500 bp indicator band at 125 ng per 5 µL; other bands 50 ng per 5 µL |
| Format | Ready-to-use; premixed in 1× Loading Buffer |
| Recommended load | 5–10 µL per well; 5 µL for wells narrower than 5 mm |
| Recommended gel | 2.5%–3% agarose for 100–500 bp; 1.5%–2% agarose for fragments above 500 bp |
Applications
- Sizing of DNA fragments in agarose gel electrophoresis
- Direct loading as a size reference alongside samples
Key Features
- Two optimized Loading Buffer dyes are added without blocking the brightness of DNA band fluorescence.
Storage & Handling
Stored at -20°C with a shelf life of two years.
Usage Notes
- Load 5 µL per run when the sample well is narrower than 5 mm; increase the volume for wider wells.
- Use 2.5%–3% agarose for fragments expected between 100 and 500 bp, and 1.5%–2% agarose for fragments expected above 500 bp.
- Agarose purity strongly affects the clarity of DNA bands, so the best quality agarose should be used.
- Higher agarose concentration improves separation of short DNA fragments, while lower concentration favours separation of long fragments.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Sequence-based analysis of Theileria annulata reveals high Tams1 gene diversity and conservation of 18 S rRNA in Türkiye. BMC Veterinary Research. 10.1186/s12917-025-05056-5
Caprine piroplasmosis: molecular epidemiology and genetic diversity in the Southeastern region of Türkiye. Tropical Animal Health and Production. 10.1007/s11250-025-04655-6
