| Field | Specification |
|---|---|
| Alternative Names | YWHAE; 14-3-3 protein epsilon; 14-3-3E |
| Clonality | |
| Concentration | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant Protein of 14-3-3 ε |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyYWHAG (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma) product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue.
Validated Applications
| Western Blot (WB) | See datasheet for recommended dilution |
|---|---|
| Immunohistochemistry (IHC) | See datasheet for recommended dilution |
Key Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Species Reactivity | Mouse, Rat |
| Isotype | IgG |
| Purification | Affinity Purification |
| Concentration | 1 mg/ml |
| Formulation | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Molecular Weight | 28303 Da |
| Catalog No. | BT-AP00007 |
| UniProt | Human: P61981; Mouse: P61982; Rat: P61983 |
| Alternative Names | YWHAE; 14-3-3 protein epsilon; 14-3-3E |
Immunogen / Antigen
Recombinant Protein of 14-3-3 ε
Reactivity Notes
This antibody has been validated for reactivity against Mouse, Rat targets. Cross-reactivity with other species may exist but has not been formally established.
Storage & Handling
-20°C for 1 year
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
All of antibodies are generated with peptides designed using the database corresponding to the primary species listed in the datasheet. Generally they are human, mouse or rat. This species was then confirmed with the western blot validation data shown for each antibody in the appropriate species. If we do not obtain positive results in one species, we do not list that species. But the cell line we test possibly contains a low level of particular protein or the inductions we used do not work in that particular cell line. We suggest you compare the peptide sequence between the species you are studying and the species that BT Lab's antibody was raised against. In most cases, BT Lab's antibodies are raised against the human protein sequence and are highly purified using affinity chromatography.
The instruction specifies the intended use for BT Lab's antibody. Any procedure not specified in the instruction should be validated by user.
Generally BT Lab offers antibodies with a purity higher than 96%.
Please store the antibody at -20°C.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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