| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C10H13N5O2 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
2',5'-Dideoxyadenosine is a potent and non-competitive adenylyl cyclase inhibitor via binding the P-site, with an IC50 of 3 μM. It is a nucleoside analog that exerts a potent antiadrenergic action in heart[1][2]. It is supplied as a white to off-white solid (C10H13N5O2, MW 235.24) at 99.86% purity.
Physical & Chemical Properties
| CAS Number | 6698-26-6 |
|---|---|
| Molecular Formula | C10H13N5O2 |
| Molecular Weight | 235.24 g/mol |
| Purity | 99.86% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | C[C@@H]1[C@H](C[C@H](N2C=NC3=C(N=CN=C32)N)O1)O |
| Signaling Pathway | GPCR/G Protein; Neuronal Signaling |
| Solubility | In Vitro: DMSO: 125 mg/mL (531.37 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
IC50: 3 μM (adenylyl cyclase)[1]
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 125 mg/mL (531.37 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | ≥ 2.5 mg/mL (10.63 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 2
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 2.08 mg/mL (8.84 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.08 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (20.8 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 2.08 mg/mL (8.84 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.08 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (20.8 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
2',5'-Dideoxyadenosine (10 μM, 30 min) lowers cAMP production and blocks carbachol (CCh)-induced phosphorylation of GluA1 at Ser845[3]. 2',5'-Dideoxyadenosine (10 μM, 30 min) blocks the CCh-induced rise in Akt phosphorylation and attenuates CCh-induced phosphorylation of Ser2448[3]. Like adenosine, 2',5'-Dideoxyadenosine (20-150 mM) inhibits, dose-dependently and reversibly, the positive inotropic and chronotropic effects of beta-adrenergic stimulation with isoproterenol (8-54 pmol), by up to 70% and 50%, respectively[2].
Western Blot Analysis[3]
| Cell Line | Primary hippocampal neurons |
|---|---|
| Concentration | 10 μM |
| Incubation Time | 30 min |
| Result | Reduced cAMP production and blocked the phosphorylation of GluA1 at Ser845 induced by carbachol (CCh). |
In Vivo
In rats, 2',5'-Dideoxyadenosine (0.1 mg/kg; IP; 15 min pre-treated) completely inhibits the diuretic, natriuretic and K+- and Cl--sparing effects of Fr EtOAc[4].
| Animal Model | Male Wistar rats (3-4 months old)[3] |
|---|---|
| Dosage | 0.1 mg/kg |
| Administration | IP; 15 min pre-treated |
| Result | Fully inhibited the diuretic, natriuretic and K+ and Cl- sparing effect of Fr•EtOAc in rats. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Melatonin Promotes the Synthesis and Secretion of Growth Hormone in the Adenohypophysis by Activating the cAMP/FOXO1 Pathway. J Pineal Res 2026 Mar;78(2):e70139. PMID: 41870323
Intracellular pathways of calcitonin gene-related peptide-induced relaxation of human coronary arteries: A key role for Gβγ subunit instead of cAMP. Br J Pharmacol 2024 Aug;181(15):2478-2491. PMID: 38583945
Tribuloside acts on the PDE/cAMP/PKA pathway to enhance melanogenesis, melanocyte dendricity and melanosome transport. J Ethnopharmacol 2024 Apr 6:323:117673. PMID: 38158096
GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion. BMC Biol 2024 May 3;22(1):104. PMID: 38702712
Res Sq. 2025 Nov 12.
