| Field | Specification |
|---|---|
| Alternative Names | H2AC11; H2AFP; HIST1H2AG;; H2AC13; H2AFC; HIST1H2AI;; H2AC15; H2AFD; HIST1H2AK;; H2AC16; H2AFI; HIST1H2AL;; H2AC17; H2AFN; HIST1H2AMHistone H2A type 1; H2A.1; Histone H2A/ptl |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Peptide sequence around site of 2-hydroxyisobutyryl-Lys (95) derived from Human Histone H2A type 1 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-HIST1H2AG antibody raised in Rabbit, tested in ELISA, IF. Designed to detect HIST1H2AG in Human for epigenetics and nuclear signaling research.
Key elements and design rationale
- Immunogen: Peptide sequence around site of 2-hydroxyisobutyryl-Lys (95) derived from Human Histone H2A type 1 — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Antigen affinity purification): Enriches Ig classes; reduces non-specific background.
Biological background
HIST1H2AG (also: H2AC11, H2AFP, HIST1H2AG,, H2AC13, H2AFC, HIST1H2AI,, H2AC15, H2AFD, HIST1H2AK,, H2AC16, H2AFI, HIST1H2AL,, H2AC17, H2AFN, HIST1H2AMHistone H2A type 1, H2A.1, Histone H2A/ptl) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current HIST1H2AG annotation relevant to epigenetics and nuclear signaling.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
- IF: Subcellular localization in fixed cells/sections; optimize fixation.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
HIST1H2AG is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects HIST1H2AG in ELISA, IF applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA, IF. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Non-conjugated IgG; requires anti-rabbit-IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin).
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing HIST1H2AG; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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