2-hydroxyisobutyryl-HIST1H3A (K36) Antibody

What it means: The target is HIST1H3A — the specific protein this antibody was raised against and designed to detect.

Why it matters:

• Confirms which analyte this antibody detects — critical for reagent selection.
• Determines epitope compatibility with your sample preparation (native vs. denatured).
• Verify aliases or gene synonyms match your experimental system before ordering.

How to interpret here: HIST1H3A is the confirmed immunogen target on this page. Confirm your system expresses HIST1H3A and that conditions are compatible with this epitope.

What it means: Reactivity of Human indicates the species in which this antibody detects the target.

Why it matters:

• Determines model organism compatibility — untested species increases risk of false negatives.
• Cross-reactivity with related species depends on epitope sequence homology and requires empirical confirmation.
• Verify species compatibility before ordering, especially for non-standard model organisms.

How to interpret here: This antibody is reported reactive against Human. Contact us for guidance on potential cross-reactivity with unlisted species.

What it means: Rabbit is the production species — the animal in which this antibody was raised — determining secondary antibody requirements.

Why it matters:

• Secondary antibodies must target Rabbit IgG; mismatched secondaries yield no signal.
• Rabbit-derived antibodies may produce elevated background in rabbit tissue samples.
• Multiplexing panels require antibodies from different host species to avoid secondary cross-reactivity.

How to interpret here: Select an anti-Rabbit IgG secondary (IgG) for indirect detection with this antibody.

What it means: Polyclonal describes whether this antibody recognizes one (monoclonal) or multiple (polyclonal) epitopes on the target.

Why it matters:

• Polyclonal: recognizes multiple epitopes; robust signal with inherent lot-to-lot variability.
• Plan lot qualification and include matching isotype controls.
• Clonality is a primary selection filter: sensitivity (polyclonal) vs. specificity/reproducibility (monoclonal).

How to interpret here: This antibody is Polyclonal. Include appropriate isotype controls and validate each lot.

What it means: Isotype IgG is the immunoglobulin class and subclass of this antibody.

Why it matters:

• Secondary antibodies, Protein A/G resins, and isotype controls must all match IgG.
• Subclass identity enables multiplexing with subclass-specific secondary reagents in the same sample.
• Some isotypes (e.g., IgM) require special handling due to size or aggregation behavior.

How to interpret here: Ensure your secondary and isotype control both specify IgG from Rabbit for valid assay results.

What it means: Non-conjugated — this antibody carries no detection label and requires a secondary antibody or detection reagent.

Why it matters:

• Non-conjugated format offers maximum flexibility — pair with any HRP, AP, fluorescent, or biotin secondary.
• Indirect detection (primary + secondary) typically amplifies signal more than direct conjugate formats.
• Ideal for multiplexing with antibodies from different host species.

How to interpret here: Select an anti-rabbit IgG (IgG) secondary with your preferred reporter for this Non-conjugated antibody.

What it means: ELISA, IF lists the assay formats in which this antibody has been tested or reported to perform.

Why it matters:

• Epitope accessibility differs by format — WB uses denatured protein; IF/flow requires native or fixed-cell conditions.
• Antibodies are not universally cross-applicable; unlisted applications require empirical testing and optimization.
• Optimal dilutions, blocking agents, and sample preparation differ significantly across formats.

How to interpret here: This antibody is reported for ELISA, IF. If your application is not listed, contact BioHippo to assess feasibility or explore custom options.

What it means: Antigen affinity purification describes how this antibody was isolated and enriched from host serum or conditioned media.

Why it matters:

• Higher-purity preparations reduce non-specific background compared to crude antisera.
• Purification grade affects concentration accuracy, formulation stability, and quantitative assay performance.
• Sensitive applications (ChIP, IP, PLA) generally benefit from higher-purity preparations.

How to interpret here: Prepared by Antigen affinity purification, providing a defined immunoglobulin fraction suitable for ELISA, IF.

What it means: Human identifies the species origin of the antigen used to immunize the host animal.

Why it matters:

• Immunogen species is the primary reactivity determinant — reliable detection is most expected in Human.
• Cross-reactivity with other species depends on epitope sequence homology; consult alignment data for untested species.
• For recombinant or synthetic immunogens, the expressed domain may differ from the full-length native protein.

How to interpret here: The immunizing antigen was derived from Human. Reactivity in other species should not be assumed without empirical testing or documented homology data.

What it means: Liquid is the physical state in which this antibody is supplied.

Why it matters:

• Liquid antibodies are ready to use after brief centrifugation; cold-chain storage must be maintained throughout.
• Repeated freeze-thaw cycles reduce activity — aliquot upon receipt to minimize this risk.
• Form affects handling, aliquoting strategy, and long-term storage stability.

How to interpret here: Supplied as Liquid. Aliquot and store at -20°C or as directed on the datasheet.