| Field | Specification |
|---|---|
| Mfr No | |
| Species |
Originating from a primary tumor of a 64-year-old female Caucasian patient diagnosed with lung squamous cell carcinoma, 2427T provides a valuable in vitro model that recapitulates the morphological traits of the original tumor tissue. Characterized by their distinctive small, round shape and propensity to aggregate into clusters, 2427T cells exhibit key morphological features typical of squamous cell carcinoma (SCC).
A defining characteristic of the 2427T cell line is its expression of cytokeratin 5/6 (CK5/6), a marker indicative of its SCC origin. The heterogeneous expression of CK5/6 hints at the presence of diverse cell subpopulations within the 2427T culture, presenting an opportunity for further exploration of intratumoral heterogeneity.
Immunophenotyping of 2427T has revealed its unique profile, including the lack of adenocarcinoma-associated marker CK7, hemato-endothelial progenitor marker CD34, and leukocyte marker CD45, reinforcing its classification within the squamous lineage. Interestingly, while the cell line generally shows negativity for neuroendocrine markers such as CD56, synaptophysin (SYP), neuron-specific enolase (NSE), and chromogranin A (CHGA), the expression of SYP in a subset of cells suggests a degree of neuroendocrine marker heterogeneity.
Crucially, the 2427T cell line does not harbor mutations in EGF-R or k-ras, distinguishing it from other models and underscoring its potential as a novel resource for delving into the biology and therapeutic vulnerabilities of squamous cell non-small cell lung cancer (NSCLC). This absence of common oncogenic mutations positions 2427T as an invaluable tool for research aimed at uncovering the underlying mechanisms of squamous cell carcinoma pathogenesis and progression.
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- Protein expression: Synaptophysin (SYP)
- Antigen expression: partial expression of CK5/6
- Tumorigenic: Highly tumorigenic in nude mice.
- cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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