| Field | Specification |
|---|---|
| Mfr No | |
| Form | SuperMix or Master Mix |
| Product Type | |
| Shipping | |
| Storage |
2× Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase, dNTPs, and other PCR-required components in an optimized ready-to-use formulation. Custom stabilizers ensure stability for 3 months at 4°C. PCR products contain a 3′-dA overhang compatible with T-vector cloning, and can be loaded directly for gel electrophoresis using the pre-loaded bromophenol blue dye.
Features
- Convenient ready-to-use 2× premix — add only template and primers
- Thermostable: half-life greater than 40 min at 95°C
- Generates PCR products with 3′-dA overhangs for TA cloning
- Pre-loaded bromophenol blue dye enables direct gel loading
- Stable for 3 months at 4°C with customized stabilizers
Applications
- Routine PCR amplification of DNA fragments
- Genotyping
- High-throughput PCR
- DNA labeling
Specifications
| Parameter | Value |
|---|---|
| Fidelity (vs. Taq) | 1× |
| Hot Start | No |
| Overhang | 3′-A |
| Polymerase | Taq DNA Polymerase |
| Reaction Format | SuperMix / Master Mix |
| Reaction Speed | Standard |
| Product Type | PCR Master Mix |
Selected Citations
[1] Han L, et al. Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration. Dev Cell. 2022;57(4):526–542. (IF: 12.270)
[2] Li Y, et al. Claudin-5a is essential for the functional formation of both zebrafish blood-brain barrier and blood-cerebrospinal fluid barrier. Fluids Barriers CNS. 2022;19(1):40. (IF: 7.662)
[3] Luo T, et al. An AAV-based NF-κB-targeting gene therapy (rAAV-DMP-miR533) to inflammatory diseases. J Inflamm Res. 2022;15:3447–3466. (IF: 6.922)
Store at −20°C; stable for up to 3 months at 4°C with the customized stabilizers included in the formulation. Avoid repeated freeze-thaw cycles. The pre-mixed format eliminates the need for separate enzyme and buffer storage.
No. The formulation contains pre-loaded bromophenol blue dye, enabling direct loading of PCR products onto agarose gels without adding additional loading buffer. This simplifies workflow and reduces contamination risk.
This mix uses Taq DNA Polymerase, which generates PCR products with 3′-dA overhangs (A-tailed ends). These products are directly compatible with TA cloning vectors and T-vector systems. They are not suitable for blunt-end cloning without additional polishing.
The Hieff™ Taq DNA Polymerase in this mix has a half-life of greater than 40 minutes at 95°C, enabling extended PCR protocols and high-cycle-number amplification without significant loss of activity.
Yes. The ready-to-use 2× format simplifies liquid handling by requiring only the addition of template and primers, making it well suited for 96-well and 384-well high-throughput PCR formats and robotic dispensing systems.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
