| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C7H4N2O7 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
3,5-Dinitrosalicylic acid is a derivative of salicylic acid. It is used in the α-amylase assay, the carbohydrase assay, and for the colorimetric determination of reducing substances[1]. It is supplied as an off-white to yellow solid (C7H4N2O7, MW 228.12) at 99.97% purity.
Physical & Chemical Properties
| CAS Number | 609-99-4 |
|---|---|
| Molecular Formula | C7H4N2O7 |
| Molecular Weight | 228.12 g/mol |
| Purity | 99.97% |
| Appearance | Solid |
| Color | Off-white to yellow |
| SMILES | O=C(O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O |
| Solubility | In Vitro: DMSO: 175 mg/mL (767.15 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | 4°C, stored under nitrogen. In solvent: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 175 mg/mL (767.15 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); stored under nitrogen; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
3,5-Dinitrosalicylic acid is a widely used reagent for detecting reducing sugars. 3,5-Dinitrosalicylic acid undergoes an oxidation-reduction reaction with reducing sugars to form derivatives, and the resulting color change allows quantitative determination of reducing sugar content. Two DNSA reagents were prepared. 1. Reagent preparation: DNSA-1 reagent: 0.4M NaOH solution, with a certain amount of heptahydrate in each 100mL. DNSA-2 reagent: 0.7M NaOH solution, with 2g 3,5-dinitrosalicylic acid in each 100mL. 2.Sample preparation: Prepare a series of maltodextrin solutions at different concentrations (0.15 - 5.85mM). To study the effect of calcium ions, add calcium chloride to the maltodextrin solution to a concentration of (40mM). 3.Test steps: Place 2mL of sample in a Folin-Wu sugar tube and add DNSA reagent. Heat in a boiling water bath for precisely 5min, then cool in cold running water for 5min. After cooling, dilute to 25mL and read absorbance on a Unicam SP 1300 colorimeter fitted with a green filter. 4.Result analysis: Plot absorbance (A) on the ordinate against the millimolar concentration of maltodextrin (M) on the abscissa. To compare molar reduction values among maltodextrins, plot the slope of each maltodextrin's absorbance-concentration curve against maltodextrin size (number of glucose residues). When reagent absorbance is proportional to the number of reducing ends, the curve slope is zero; when absorbance is not proportional to the number of reducing ends and rises with maltodextrin size, the slope is greater than zero.
Data provided by the manufacturer.
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