| Field | Specification |
|---|---|
| Applications | |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Assay time | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 3-NT. During the reaction, Universal 3-NT in the sample or standard competes with a fixed amount of Universal 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 0.94 ng/mL |
|---|---|
| Detection Range | 1.56-100 ng/mL |
| Total Assay Time | 2 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Universal (multi-species validated) |
| Detection Method | Competitive |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Universal 3-NT in samples.No significant cross-reactivity or interference between Universal 3-NT and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 0.94 ng/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 2 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Competitive ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant 3-NT standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
What is the range of enzyme activity of your IL-2 freeze-dried powder
What is the principle of adding stop solution to stop color reaction in ELISA experiment?
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Ratiometric SERS imaging for indication of peroxynitrite fluctuations in diabetic wound healing process (2023) CHEMICAL ENGINEERING JOURNAL. 10.1016/j.cej.2023.144024
Ambient fine particulate matter exposures and oxidative protein damage in early pregnant women (2022) ENVIRONMENTAL POLLUTION. 10.1016/j.envpol.2022.120604
Attenuated effect of zinc gluconate on oxidative stress, inflammation, and angiogenic imbalance in pre-eclampsia rats (2022) LIFE SCIENCES. 10.1016/j.lfs.2022.121055
Protection against Doxorubicin-Induced Cardiotoxicity through Modulating iNOS/ARG 2 Balance by Electroacupuncture at PC6 (2021) Oxidative Medicine and Cellular Longevity. 10.1155/2021/6628957
Iron Replacement and Redox Balance in Non-Anemic and Mildly Anemic Iron Deficiency COPD Patients: Insights from a Clinical Trial (2021) Biomedicines. 10.3390/biomedicines9091191
Phytochemical Composition Antioxidant and Anti-Inflammatory Activity of Artemisia dracunculus and Artemisia abrotanum (2024) Antioxidants. 10.3390/antiox13081016
Phytochemical Profile and Antidiabetic, Antioxidant, and Anti-Inflammatory Activities of Gypsophila paniculata Ethanol Extract in Rat Streptozotocin-Induced Diabetes Mellitus (2024) Antioxidants. 10.3390/antiox13091029
Direct detection of 3-nitrotyrosine reveals the nitration of proteins in laboratory exposure and ambient aerosols (2024) Journal of Environmental Sciences. 10.1016/j.jes.2024.06.009
Nitrosative and Oxidative Stress, Reduced Antioxidant Capacity, and Fiber Type Switch in Iron-Deficient COPD Patients: Analysis of Muscle and Systemic Compartments (2023) Nutrients. 10.3390/nu15061454
Neuroprotection Against NMDA-Induced Retinal Damage by Philanthotoxin-343 Involves Reduced Nitrosative Stress (2021) Frontiers in Pharmacology. 10.3389/fphar.2021.798794
Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species (2022) Metabolites. 10.3390/metabo13010049
Association of hydroxytyrosol enriched olive oil with vascular function in chronic coronary disease (2023) EUROPEAN JOURNAL OF CLINICAL INVESTIGATION. 10.1111/eci.13983
Therapeutic potential of alpha-lipoic acid on mitochondrial dynamics, oxidative/nitrosative stress, and histopathological changes in rat ulcerative colitis model (2025) INFLAMMOPHARMACOLOGY. 10.1007/s10787-025-01918-4
Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression (2020) Oxidative Medicine and Cellular Longevity. 10.1155/2020/7106525
The Phytochemical and Functional Characterization of the Aerial Parts of Artemisa alba Turra (Asteraceae) Grown in Romania (2025) Foods. 10.3390/foods14081389
White Teff Flour Ethanolic Extract: Phytochemical Profile, Antioxidant and Anti-Inflammatory Activity (2026) MOLECULES. 10.3390/molecules31040644
Gluten-Free White Quinoa Flour Exhibits Antioxidant and Anti-Inflammatory Activity (2026) MOLECULES. 10.3390/molecules31040736
Whole-Grain Oryza sativa L. Flour Extract Exhibits Potent Antioxidant and Anti-Inflammatory Activity in Rats with Experimentally Induced Inflammation (2026) MOLECULES. 10.3390/molecules31061012
The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice (2024) iScience. 10.1016/j.isci.2024.109134
Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study (2021) Scientific Reports. 10.1038/s41598-021-91256-6
Aesculus hippocastanum Extract Exerts Neuroprotective Effects in an MPP+‐Induced Parkinson's Disease Model via PPARγ Activation (2026) JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 10.1111/jcmm.71006
Modulation of Wnt/β-Catenin Pathway by Aesculus hippocastanum Extract Enhances Temozolomide Sensitivity in Glioblastoma Cells (2026) JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 10.1111/jcmm.70979
Flowers and Leaves of Artemisia absinthium and Artemisia annua Phytochemical Characterization, Anti-Inflammatory, Antioxidant, and Anti-Proliferative Activities Evaluation (2025) Plants-Basel. 10.3390/plants14071029
Assessment of Opuntia ficus-indica supplementation on enhancing antioxidant levels (2025) Scientific Reports. 10.1038/s41598-025-87680-7
P66Shc is increased in peripheral blood mononuclear cells of the patients with obstructive sleep apnea (2023) International Journal of Medical Sciences. 10.7150/ijms.80343
