| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Hygromycin, Puromycin, RFP (constitutively expressed), Zeocin |
| Shipping | |
| Species |
Background
4-1BB (CD137, TNFRSF9) is a co-stimulatory receptor of the tumor necrosis factor receptor superfamily, induced on activated T cells and natural killer cells. Engagement by its ligand 4-1BBL delivers a powerful co-stimulatory signal that enhances T cell activation, proliferation, survival, and the formation of memory populations. 4-1BB signaling proceeds through TRAF adaptors to activate downstream pathways, and amplifies antigen-receptor-driven NFAT-dependent transcription. Because of its ability to boost effector responses, the 4-1BB axis is a major target for agonist immunotherapies and is incorporated as a co-stimulatory domain in chimeric antigen receptor designs. NFAT reporters provide a quantitative readout of 4-1BB-enhanced T cell activation.
Product Description & Applications
The 4-1BB/NFAT Reporter Lentivirus is an all-in-one immunotherapy reporter system for studying 4-1BB signaling in T cells. The construct constitutively expresses the human 4-1BB receptor and couples its engagement to NFAT-dependent transcription of dual reporters: secreted Gaussia luciferase and a fluorescent protein (GFP or RFP), enabling real-time monitoring of NFAT activation by luminometry and fluorescence.
Transducing target cells with the high-titer lentivirus establishes a stable, pathway-specific reporter cell line. Applications include evaluating co-stimulatory signaling, testing agonistic antibodies, and studying immune activation pathways. Supplied as third-generation, VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, the product performs well in difficult-to-transfect primary and thawed cells.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human 4-1BB under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
