| Field | Specification |
|---|---|
| Alternative names | 4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C22H35NO4 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
4-Nitrophenyl palmitate, also known as 4-Nitrophenyl hexadecanoate, p-Nitrophenyl Palmitate, or pNpp, is a chromogenic substrate for lipases and esterases. Enzymatic hydrolysis releases p-nitrophenol from the compound, which can then be measured by colorimetric detection at 410 nm as an indicator of enzymatic activity. It is used to characterize enzymes from various bacterial and mammalian sources, including Burkholderia species and porcine pancreatic lipase[1][2]. It is supplied as a white to off-white solid (C22H35NO4, MW 377.52) at 99.22% purity.
Physical & Chemical Properties
| CAS Number | 1492-30-4 |
|---|---|
| Molecular Formula | C22H35NO4 |
| Molecular Weight | 377.52 g/mol |
| Purity | 99.22% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | CCCCCCCCCCCCCCCC(OC1=CC=C([N+]([O-])=O)C=C1)=O |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Solubility | In Vitro: Chloroform: ≥ 100 mg/mL (264.89 mM) DMSO: 6.67 mg/mL (17.67 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) * "≥" means soluble, but saturation unknown. |
| Storage | 4°C, protect from light. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| Chloroform | ≥ 100 mg/mL (264.89 mM) | — |
| DMSO | 6.67 mg/mL (17.67 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); protect from light; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
4-Nitrophenyl palmitate is a substrate for measuring the esterolytic activity of porcine pancreatic lipase, Staphylococcus aureus protease, and Burkholderia sp. lipase, using colorimetric detection of the p-nitrophenol released; adding Triton X-100 to the reaction mixture resolves turbidity problems[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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