4-ONE

Product details

• Chemical name: (E)-4-oxonon-2-enal.
• Also known as: 4-Oxo-2-nonenal
• CAS number: 103560-62-9
• Molecular formula: C9H14O2.
• Purity: >97%
• Concentration: 1-100 µM.
• Form: Lyophilized powder.
• Solubility: Supplied in oil form. Soluble in DMSO, methanol, ethanol.Centrifuge all products before use (10000 x g 5 min).
• Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: TRPA1 channels
• Source: Synthetic
• Activity: 4-ONE (4-Oxo-2-nonenal) is a lipid peroxidation product with multiple biological activities, shown to activate TRPA1 channels (EC50 = 1.9 µM)1.
• Alternative names: 4-Oxo-2-nonenal

Scientific background

4-ONE is a lipid peroxidation product that acts as a TRPA1 (transient receptor potential ankyrin 1) channel activator and displays an EC50 value of 1.9 µM1. The compound contains an electrophilic unsaturated carbonyl moiety and forms adducts with lysine, histidine, and cysteine residues1. 4-ONE is rich in β-sheet structures and is considered to be a reactive aldehyde that may be also cytotoxic2.A study conducted with 4-ONE showed that the compound is able to trigger unilateral mechanical hyperalgesia and vasodilatation in mice and also triggersTRPA1-independent effects that relate to edema formation and pain3.TRPA1 is a non-selective cation channel expressed in the small neurons of the dorsal root and trigeminal ganglia. TRPA1 channels play an important role in models of inflammatory and neuropathic pain and have the ability to detect pungent or irritant compounds4.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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• Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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