| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C4H4N2OS |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
4-Thiouracil is a thionucleobase with cytostatic properties that can be used as a biological photoprobe to detect RNA structures and nucleic acid–nucleic acid contacts. It also acts as a strong ultraviolet A (UVA) photosensitizer, generating reactive oxygen species such as O2, and can be used in research on photocrosslinking, photodamage, and photodynamic therapy[1]. It is supplied as a light yellow to yellow solid (C4H4N2OS, MW 128.15) at 99.71% purity.
Physical & Chemical Properties
| CAS Number | 591-28-6 |
|---|---|
| Molecular Formula | C4H4N2OS |
| Molecular Weight | 128.15 g/mol |
| Purity | 99.71% |
| Appearance | Solid |
| Color | Light yellow to yellow |
| SMILES | O=C(N1)NC=CC1=S |
| Signaling Pathway | Immunology/Inflammation; NF-κB; Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 50 mg/mL (390.17 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Store at room temperature 3 years. In solvent: -80°C, 2 years; -20°C, 1 year. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 50 mg/mL (390.17 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 2 years) or -20°C (up to 1 year); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | 5 mg/mL (39.02 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 5 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (50.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Data provided by the manufacturer.
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