| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | 200 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 5-chloro-1H-indole.
- CAS number: 17422-32-1
- Molecular formula: C8H6ClN.
- Purity: >98%
- Concentration: 1-100 µM.
- Form: Lyophilized powder.
- Solubility: 200 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: 5-HT3 receptors
- Source: Synthetic
- Activity: 5-Chloroindole is a potent and selective allosteric modulator of the 5-HT3 receptor that displays micromolar potency1.
Peptide confirmation
Confirmed by amino acid analysis and mass spectrometry
Scientific background
5-Chloroindole is a potent and selective allosteric modulator of the 5-HT3 receptor that displays micromolar potency.The 5-HT3 receptor belongs to the Cys-loop ligand-gated ion channel superfamily. It mediates fast synaptic neurotransmission in neurons of both the central and peripheral nervous systems.5-Chloroindole can assist in the investigation of the molecular basis for allosteric modulation of the 5-HT3 receptor and may contribute to the discovery of novel therapeutic drugs targeting the 5-HT3 receptor1. 5-Chloroindole is also used as a strong inhibitor of the copper dissolution in acidic sodium chloride solution2. Several different studies have shown that 5-chloroindole has the ability to decrease serotonin levels in the brainstem and in the telencephalon3.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
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