5-Fluorouracil-1-yl  acetic acid methyl ester

What it means: This compound is classified as a Small Molecule Compound — a small molecule that modulates the activity of its target through direct binding.

Why it matters:

• The activity class tells you how this compound interacts with its target: as an inhibitor, agonist, or antagonist — determining appropriate experimental controls and expected phenotypic outcomes.
• Knowing the class is essential for dose-response design, positive control selection, and correct interpretation of cell viability or reporter assay results.

How to interpret here: Small Molecule Compound indicates this compound modulates its molecular target. Confirm the mechanism (competitive, allosteric, or covalent) in the DataSheet before designing washout or time-point experiments.

What it means: CAS 56059-28-0 is the unique Chemical Abstracts Service registry number that unambiguously identifies this compound across databases and literature.

Why it matters:

• CAS numbers allow cross-referencing in PubChem, ChEMBL, and SciFinder to retrieve verified physicochemical data, toxicology profiles, and published experimental concentrations.
• Specifying CAS 56059-28-0 in methods ensures replication teams can source the identical compound regardless of trade name or vendor differences.

How to interpret here: CAS 56059-28-0 confirms chemical identity independent of batch or supplier. Verify against your institutional chemical registry before ordering.

What it means: The Molecular Weight is 202.14 g/mol — the mass of one mole of this compound in grams.

Why it matters:

• MW is required to calculate molar concentration: essential for comparing IC₅₀ or EC₅₀ values with published molar data.
• MW provides a quick indication of drug-likeness — most cell-permeable compounds are below 500 g/mol (Lipinski Rule of Five).

How to interpret here: To make a stock solution, use: [mM] = (mass [mg] × 1000) / (MW × volume [mL]). Example: 5 mg in 1 mL DMSO = 24.7 mM at MW 202.14 g/mol.

What it means: This compound is supplied as a Solid — typically a lyophilized powder or crystalline material requiring dissolution before use.

Why it matters:

• Solid format offers superior long-term stability versus pre-dissolved solutions, eliminating solvent-related degradation and concentration drift during storage.
• You control solvent choice (DMSO, EtOH, aqueous) and concentration to suit your assay.

How to interpret here: Prepare a 10–50 mM stock in DMSO, then dilute into aqueous buffer to ≤0.1% DMSO final. Consult the DataSheet for solubility guidance before preparing aqueous stocks without a co-solvent.

Tip: Check logP or SMILES before assuming water solubility. Most small molecule inhibitors require DMSO for stock preparation.

What it means: This compound ships at Room Temperature (RT), approximately 18–25°C, without cold-chain packaging.

Why it matters:

• RT shipment simplifies procurement — no dry ice scheduling or temperature monitoring required.
• The supplier confirms stability through standard ambient transit conditions.

How to interpret here: Although shipped at RT, store at −20°C immediately upon receipt. RT shipment applies to transit only and does not indicate suitability for room-temperature storage.