| Field | Specification |
|---|---|
| Target | |
| Alternative names | 6*His|6*His|His Tag|6histag|6XHis|HHHHHH|HIS|His Tag|HISTIDINE TAGGED |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Product Overview
This monoclonal antibody (clone 0B1), raised in Mouse, recognizes 6*His, His-Tag. It is validated for ELISA, Western Blot.
Validated Applications
| Applications | ELISA, Western Blot |
|---|---|
| WB | 1:500-1:2000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A synthetic peptide
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 0B1 |
| Isotype | IgG2b |
| Conjugate | Unconjugated |
| Reactivity | Recombinant Protein |
| Immunogen | A synthetic peptide |
| Purification | Protein A+G Purification |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | 6*His|6*His|His Tag|6histag|6XHis|HHHHHH|HIS|His Tag|HISTIDINE TAGGED |
Reactivity Notes
Validated reactivity: Recombinant Protein. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for 6*His, His-Tag research from Fine Test, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
