| Field | Specification |
|---|---|
| Alternative names | 8-Azidoadenosine 5'-triphosphate; 8-N3-ATP |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C10H15N8O13P3 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
8-Azido-ATP, also known as 8-Azidoadenosine 5'-triphosphate or 8-N3-ATP, is a photoreactive nucleotide analog useful for identifying proteins such as DNA-dependent RNA polymerase[1]. It is a click chemistry reagent bearing an azide group that can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with alkyne-containing molecules, as well as strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules bearing DBCO or BCN groups. It has the molecular formula C10H15N8O13P3 and a molecular weight of 548.19 g/mol.
Physical & Chemical Properties
| CAS Number | 53696-59-6 |
|---|---|
| Molecular Formula | C10H15N8O13P3 |
| Molecular Weight | 548.19 g/mol |
| SMILES | O[C@H]1[C@@H](O)[C@H](N2C(N=[N+]=[N-])=NC3=C(N=CN=C32)N)O[C@@H]1COP(O)(OP(OP(O)(O)=O)(O)=O)=O |
| Signaling Pathway | Membrane Transporter/Ion Channel |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
8-Azido-ATP can serve to identify the viral RNA polymerase, since the analog inhibits transcription activity associated with rotavirus particles after UV light exposure[1]. 8-Azido-ATP (azido-ATP) lowers viral transcription in a dose-dependent manner[1]. 8-Azido-ATP blocks Kir6.2ΔC26 currents less potently than ATP does: its half-maximal inhibition (Ki) is 2.8 ± 0.4 mM (n = 6), against 172 ± 7 mM (n = 6) for ATP. The Hill coefficients were 0.9 ± 0.2 (8-azido-ATP) and 1.3 ± 0.1 (ATP)[2].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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