| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Source | Plant — Scrophulariaceae Picria felterrae Lour. |
| Molecular weight | |
| Molecular formula | C15H16O4 |
| Purity | |
| SMILES | |
| Form | Oil |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
8-Deoxylactucin is an orally active sesquiterpene lactone that inhibits LPS-induced NO production in RAW264.7 macrophages with an IC50 of 4.35 μM and exerts anti-inflammatory effects through blockade of the NF-κB pathway. It also shows hepatoprotective efficacy in a mouse model of LPS/D-galactosamine-induced acute hepatitis, supporting its use in studies of inflammatory disease and inflammatory liver injury[1]. It is supplied as an off-white to light brown oil (C15H16O4, MW 260.29) at 95.0% purity.
Physical & Chemical Properties
| CAS Number | 65725-10-2 |
|---|---|
| Molecular Formula | C15H16O4 |
| Molecular Weight | 260.29 g/mol |
| Purity | 95.0% |
| Appearance | Oil |
| Color | Off-white to light brown |
| Structure Classification | Terpenoids Sesquiterpenes |
| SMILES | OCC([C@]1([H])[C@]2([H])[C@](CCC(C)=C13)([H])C(C(O2)=O)=C)=CC3=O |
| Signaling Pathway | NF-κB; Metabolic Enzyme/Protease; Immunology/Inflammation |
| Initial Source | Plant — Scrophulariaceae Picria felterrae Lour. |
| Storage | Pure form: -20°C, 3 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
In RAW264.7 macrophages, 8-Deoxylactucin (1 h pretreatment) significantly suppresses LPS-induced NO production, yielding an IC50 of 4.35 μM[1]. At 1-10 μM and 1 h pretreatment, 8-Deoxylactucin significantly lowers iNOS mRNA and protein expression induced by LPS, along with mRNA expression of the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α, in RAW264.7 cells[1]. In RAW264.7 macrophages, 8-Deoxylactucin (1-10 μM, 2 h pretreatment) significantly prevents LPS-induced phosphorylation of IKKα/β and IκBα, inhibits IκBα degradation, and lowers nuclear accumulation of NF-κB p65[1]. In RAW264.7 cells, 8-Deoxylactucin (1-10 μM, 1 h pretreatment) significantly increases protein expression and nuclear translocation of NRF2, up-regulates protein expression of the NRF2-targeted antioxidant genes (HO, NQO1), and lowers ROS production[1].
Real Time qPCR[1]
| Cell Line | RAW264.7 macrophages |
|---|---|
| Concentration | 1, 5, 10 μM |
| Incubation Time | 1, 2 h pretreatment |
| Result | Reduced the mRNA expression of pro-inflammatory cytokines including IL-1β, IL-6, and TNF-α in RAW264.7 cells. |
Western Blot Analysis[1]
| Cell Line | RAW264.7 macrophages |
|---|---|
| Concentration | 1, 5, 10 μM |
| Incubation Time | 1, 2 h pretreatment |
| Result | Reduced LPS-induced expression of iNOS. Blocked LPS-induced phosphorylation of IKKα/β and IκBα. Inhibited IκBα degradation. Reduced nuclear accumulation of NF-κB p65. |
In Vivo
In mice, 8-Deoxylactucin (2-10 mg/kg, i.g., once daily, 5 days) significantly lowers the LPS/D-galactosamine (D-GalN)-induced rise in liver index (liver weight/body weight)[1].
| Animal Model | LPS/D-galactosamine (D-GalN) (LPS: 50 μg/kg, D-GalN: 700 mg/kg, i.p. injection) was administered to 6-week-old male ICR mice (22-24 g) to induce acute hepatitis model[1] |
|---|---|
| Dosage | 2, 5, 10 mg/kg |
| Administration | i.g., once daily, 5 days |
| Result | Achieved significant reduction in liver index. Showed significant alleviation of liver tissue damage and decreased pathological scores. Reduced serum ALT and AST levels. Decreased protein expression of IL-1β, IL-6, TNF-α, and 4-HNE in liver tissues. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).
