96-Well Anionic Protein-Binding Plates

What it means: 96-Well Plate — the plate format used for the binding reactions.

Why it matters:

• 96-well plates are read in a standard microplate reader and fit multichannel pipettes and plate washers.
• Each well holds one binding reaction, so many conditions, partners or concentrations can be compared side by side.
• The 96-well layout leaves room for blanks and controls, such as wells without the first or the second molecule, on the same plate.
• The ProFoldin protocol uses 200-300 µl per well for binding, blocking and rinsing.

For this product: This product is supplied as 96-Well Plates in packs of 4, in black or transparent.

Tip: Plan the plate map before coating, so blank and control wells sit next to the samples they belong to.

What it means: Black or Transparent — the color of the plate body, which sets the type of optical readout.

Why it matters:

• Black plates absorb stray light, which keeps background low for fluorescence detection.
• Transparent plates let light pass through the well, as needed for absorbance readings.
• Using a black plate for absorbance, or a transparent plate for fluorescence, gives weak or noisy signals.
• Keeping the same plate color across a study makes results easier to compare.

For this product: Black or Transparent: both colors share the same binding surface and plate format and are sold as separate options of this product.

Tip: Choose black for fluorescence or transparent for absorbance readouts, and keep blank wells on every plate to measure background.

What it means: 4 plates — how many plates come in one pack.

Why it matters:

• Four plates give 384 wells for binding reactions, blanks and controls.
• Coating and blocking conditions can be optimized on one plate before the main experiment.
• The black and transparent options contain the same number of plates.
• Black and transparent packs can be ordered together when both readouts are needed.
• Plates are used one at a time, so unused plates can stay in their packaging until needed.

For this product: Each pack of this product contains 4 plates of the selected color. Each plate has 96 wells, and each well holds one binding reaction.

Tip: Set aside one plate for optimizing coating concentration and blocking before running the main samples.

What it means: Negatively Charged Proteins — what the plate surface captures.

Why it matters:

• The surface binds negatively charged proteins, so the bound protein can act as bait for a second molecule.
• A protein is negatively charged when the buffer pH is above its isoelectric point (pI).
• Bound proteins often keep their native conformation and their catalytic or binding activity.
• The blocker is chosen by the second molecule: 1% BSA for proteins, Nucleic Acid Binding Blocker for nucleic acids.

For this product: These plates bind negatively charged proteins. Coat 200 µl of 0.5-1 mg/ml protein per well in a low-salt buffer at 4°C overnight.

Tip: Check the pI of your protein and the pH of the coating buffer before choosing between the anionic and cationic plates.

What it means: Polystyrene — the material the plates are made from.

Why it matters:

• Polystyrene binds proteins well and gives low background in optical reads.
• Polystyrene is the common material for microplates used in ELISA and binding assays.
• Organic solvents can damage polystyrene, so aqueous buffers are used for coating, blocking and washing.
• Polystyrene plates fit standard microplate readers, plate washers and multichannel pipettes.
• Recording the plate material in your methods helps others repeat your binding conditions.

For this product: Polystyrene: the same material is used for the black and the transparent options of this product.

Tip: Check that any solvents or detergents in your samples are compatible with polystyrene plates, and record the plate material in your methods.

What it means: Room Temperature — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Keeping the shipping box until the contents are checked makes any claim easier.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.

For this product: Room Temperature: Shipped at room temperature. Please contact us for storage conditions.

Tip: Open and check the package on arrival, and contact us right away if anything is damaged or missing.