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This kit measures the DNA decatenation activity of E. coli gyrase, which converts concatenated DNA into decatenated DNA, in a 96-well format.
Kit Components
| Component | T2DD-96KE |
|---|---|
| Assay buffer | Included |
| Concatenated DNA | Included |
| E. coli gyrase | Included |
| ATP | Included |
| Fluorescence dye | Included |
| Rinse buffer | Included |
| TDD filter plate | 1 plate |
Specifications
| Assay Target | E. coli gyrase DNA decatenation |
|---|---|
| Target Type | DNA Topoisomerase II (Gyrase) |
| Organism | E. coli |
| Assay Type | Enzyme Activity Assay |
| Detection Method | Fluorescence |
| Plate Format | 96-well filter plate |
| Size | 96 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this E. coli gyrase DNA decatenation assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.