| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Plate format | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Drug Target Assay Kit
Gyrase is a bacterial type II DNA topoisomerase. This kit tests the formation of P. aeruginosa gyrase DNA cleavage complexes in a 96-well format.
Kit Components
| Component | T2C96KP |
|---|---|
| 10 x Reaction buffer | 300 µl |
| 10 x DNA | 30 µl |
| SDS solution | 260 µl |
| 100 mM ATP | 30 µl |
| Proteinase K solution | 260 µl |
| TDC matrix | 10 ml |
| Rinse buffer | 6 ml |
| 10 x Dye | 500 µl |
| Black receiver plate | 1 plate |
| V-bottom reaction plate | 1 plate |
| TDC filter plate | 1 plate |
| 100 x P. aeruginosa gyrase | 26 µl |
A plate filtration device and a vacuum line are needed for the filtration step (not included).
Specifications
| Assay Target | P. aeruginosa gyrase DNA cleavage |
|---|---|
| Target Type | DNA Topoisomerase II (Gyrase) |
| Organism | P. aeruginosa |
| Assay Type | Enzyme Activity Assay |
| Plate Format | 96-well filter plate |
| Size | 96 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The 96-Well P. aeruginosa Gyrase DNA Cleavage Assay Kit Plus is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
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Q.What does this kit measure?
A.It measures DNA cleavage complex formation by P. aeruginosa gyrase in a 96-well filter-plate format, so compounds that trap the enzyme on cut DNA can be identified.
Q.What is included in the kit?
A.The 96-Well P. aeruginosa Gyrase DNA Cleavage Assay Kit Plus (96 assays) includes 10 x reaction buffer (300 µl), 10 x DNA (30 µl), SDS solution (260 µl), 100 mM ATP (30 µl), proteinase K solution (260 µl), TDC matrix (10 ml), rinse buffer (6 ml), 10 x dye (500 µl), black receiver plate (1 plate), V-bottom reaction plate (1 plate), TDC filter plate (1 plate) and 100 x P. aeruginosa gyrase (26 µl).
Q.Is a protocol available?
A.A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Q.How should the kit be stored?
A.The 96-Well P. aeruginosa Gyrase DNA Cleavage Assay Kit Plus is shipped frozen. Please contact us for long-term storage conditions.
Can’t find the exact format you need? We can help you source or customize this P. aeruginosa gyrase DNA cleavage assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
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