96-Well S. aureus Gyrase DNA Decatenation Assay Kit Plus

SKU:BHT16300066
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Overview
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Fluorescence-based 96-well kit for measuring the DNA decatenation activity of S. aureus gyrase. Concatenated DNA serves as substrate, reactions are stopped with EDTA, processed on a TDD filter plate and read with a fluorescence dye. S. aureus gyrase included; one 96-assay size.
Assay Target S. aureus gyrase DNA decatenation
Detection Method Fluorescence
Plate Format 96-well filter plate
Options selector
Catalog no. Size
T2DD-96KS 96 assays
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 96 assays
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped frozen.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Organism S. aureus
Applications
  • Enzyme Activity
Assay type
  • Enzyme Activity Assay
Assay target S. aureus gyrase DNA decatenation
Assay area Antibacterial Drug Discovery
Target type DNA Topoisomerase II (Gyrase)
Detection method
  • Fluorescence
Plate format 96-well filter plate
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.
Catalog no. (Mfr.) T2DD-96KS
Main SKU BHT16300066
Drug Target Assay Kit

This kit measures the DNA decatenation activity of S. aureus gyrase, which converts concatenated DNA into decatenated DNA, in a 96-well format.

Kit Components

Component T2DD-96KS
10 x Assay buffer 600 µl
10 x Concatenated DNA 500 µl
10 mM ATP 120 µl
50 x S. aureus gyrase 100 µl
Stop solution (0.4 M EDTA) 600 µl
10 x Fluorescence dye 500 µl
10 x Rinse buffer 2 ml
V-bottom plate 1 plate
TDD filter plate 1 plate
Black 96-well plate 1 plate

Specifications

Assay Target S. aureus gyrase DNA decatenation
Target Type DNA Topoisomerase II (Gyrase)
Organism S. aureus
Assay Type Enzyme Activity Assay
Detection Method Fluorescence
Plate Format 96-well filter plate
Size 96 assays
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.

Protocol

A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The 96-Well S. aureus Gyrase DNA Decatenation Assay Kit Plus is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
Q.What does this kit measure?
A.It measures the DNA decatenation activity of S. aureus gyrase, using concatenated DNA as substrate and a fluorescence dye readout in a 96-well filter-plate format.
Q.What is included in the kit?
A.The 96-Well S. aureus Gyrase DNA Decatenation Assay Kit Plus (96 assays) includes 10 x assay buffer (600 µl), 10 x concatenated DNA (500 µl), 10 mM ATP (120 µl), 50 x S. aureus gyrase (100 µl), stop solution (0.4 M EDTA) (600 µl), 10 x fluorescence dye (500 µl), 10 x rinse buffer (2 ml), V-bottom plate (1 plate), TDD filter plate (1 plate) and black 96-well plate (1 plate).
Q.Is a protocol available?
A.A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Q.How should the kit be stored?
A.The 96-Well S. aureus Gyrase DNA Decatenation Assay Kit Plus is shipped frozen. Please contact us for long-term storage conditions.

Can’t find the exact format you need? We can help you source or customize this S. aureus gyrase DNA decatenation assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today