| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Detection method | |
| Plate format | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
This kit measures the DNA decatenation activity of S. pneumoniae topoisomerase IV, which converts concatenated DNA into decatenated DNA, in a 96-well format.
Kit Components
| Component | NDD96KE |
|---|---|
| 10 x Assay buffer | 600 µl |
| 10 x Concatenated DNA | 500 µl |
| 10 mM ATP | 110 µl |
| 1000 x S. pneumoniae topo IV | 8 µl |
| 0.4 M EDTA | 600 µl |
| 20 x Fluorescence dye | 260 µl |
| 10 x Rinse buffer | 2 ml |
| V-bottom plate | 1 plate |
| TDD filter plate | 1 plate |
| Black 96-well plate | 1 plate |
Specifications
| Assay Target | S. pneumoniae Topo IV decatenation |
|---|---|
| Target Type | DNA Topoisomerase IV |
| Organism | S. pneumoniae |
| Assay Type | Enzyme Activity Assay |
| Detection Method | Fluorescence |
| Plate Format | 96-well filter plate |
| Size | 96 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this S. pneumoniae Topo IV decatenation assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.