| Field | Specification |
|---|---|
| Mfr No | |
| Species |
A427 cells originate from lung tissue, specifically a carcinoma, exhibit epithelial morphology and grow adherently. A427 cells have a doubling time of approximately 28 hours in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS).
In ACL-3 medium, the doubling time is slightly extended to 38 hours, while in ACL-3 supplemented with bovine serum albumin (BSA), it reaches 42 hours. These variations in doubling time provide valuable insights into cell behaviour under different experimental conditions.
At passage 60, A427 cells display a hypotriploid to hypertriploid karyotype. This means the cells possess abnormal chromosomes, including dicentrics, minutes, and a large subtelocentric marker. Such karyotypic abnormalities are often associated with cancer cells and contribute to the unique characteristics of this cell line. A427 cells exhibit tumorigenic properties, allowing them to form tumours when injected into nude mice.
These tumours resemble undifferentiated adenocarcinoma, further emphasizing the relevance of this cell line in studying lung cancer and its progression. With its exceptional features, A427 cells find utility in various applications, particularly in cancer research. Their epithelial morphology and lung origin make them an ideal model for studying lung cancer and related diseases. Additionally, A427 cells are well-suited for 3D cell culture techniques, providing a more physiologically relevant environment to explore the behaviour of lung cancer cells.
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- Protein expression: p53 positive
- Tumorigenic: Yes, in nude mice. Forms an undifferentiated tumor suggestive of adenocarcinoma.
- Karyotype: P60) hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2 will result in a confluent monolayer within 3 days.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 4 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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