| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human ACBD3 was used as the immunogen for the ACBD3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ACBD3 Antibody / Acyl-CoA binding domain containing protein 3 is a anti-ACBD3 Rabbit antibody Recombinant Rabbit Monoclonal clone 32A12 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: ACBD3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32A12, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Acyl-CoA binding domain containing protein 3 contains multiple domains that enable interaction with diverse partners, including lipid modifying enzymes, Golgi structural proteins, and viral proteins. Research using ACBD3 antibody has shown that it recruits phosphatidylinositol 4 kinase beta to the Golgi, controlling synthesis of phosphoinositides required for membrane trafficking. This positions ACBD3 as a central regulator of Golgi homeostasis and lipid signaling, with broad implications for secretory pathway biology.
Beyond cellular homeostasis, ACBD3 has been identified as a host factor for picornaviruses, including enteroviruses and coxsackieviruses. Studies with ACBD3 antibody have demonstrated that viral proteins hijack ACBD3 to recruit lipid kinases and remodel Golgi membranes for replication organelle formation. This makes ACBD3 an important factor in viral pathogenesis and a potential antiviral target. By detecting Acyl-CoA binding domain containing protein 3, researchers can explore how viruses exploit host cell machinery to establish infection.
In addition to viral infection, ACBD3 contributes to steroidogenesis, mitochondrial communication, and cancer biology. Research with ACBD3 antibody has revealed that altered expression of ACBD3 influences cholesterol trafficking and steroid hormone synthesis. Its interaction with protein kinases and signaling complexes also links it to cell proliferation and oncogenic signaling. Because of its broad interaction network, ACBD3 is emerging as a key integrator of metabolic and signaling pathways.
ACBD3 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting demonstrates expression in multiple tissues, immunohistochemistry highlights localization in endocrine and epithelial organs, and immunofluorescence shows Golgi specific staining. These applications make ACBD3 antibody valuable for studies of organelle biology, lipid metabolism, and infection.
By supplying validated ACBD3 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
