| Field | Specification |
|---|---|
| Alternative names | Acetyl-CoA trisodium |
| CAS no. | |
| Applications | |
| Source | Endogenous metabolite |
| Molecular weight | |
| Molecular formula | C23H35N7Na3O17P3S |
| Purity | |
| Activity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Acetyl Coenzyme A trisodium (Acetyl-CoA trisodium) is a membrane-impermeant central metabolic intermediate that participates in the TCA cycle and oxidative phosphorylation. It regulates various cellular mechanisms by serving as the sole donor of acetyl groups to target amino acid residues in post-translational protein acetylation, and it is also a key precursor of lipid synthesis[1][2][3][4]. It is supplied as a white to off-white solid (C23H35N7Na3O17P3S, MW 875.52) at 99.22% purity.
Physical & Chemical Properties
| CAS Number | 102029-73-2 |
|---|---|
| Molecular Formula | C23H35N7Na3O17P3S |
| Molecular Weight | 875.52 g/mol |
| Purity | 99.22% |
| Appearance | Solid |
| Color | White to off-white |
| Structure Classification | Others |
| SMILES | O=P(OCC([C@@H](O)C(NCCC(NCCSC(C)=O)=O)=O)(C)C)(O)OP(OC[C@@H]1[C@@H](OP(O[Na])(O[Na])=O)[C@@H](O)[C@H](N2C3=NC=NC(N)=C3N=C2)O1)(O[Na])=O |
| Signaling Pathway | Metabolic Enzyme/Protease; Autophagy |
| Bioactivity Class | Human Endogenous Metabolite |
| Initial Source | Endogenous metabolite |
| Solubility | In Vitro: H2O: 83.33 mg/mL (95.18 mM; Requires sonication) |
| Storage | 4°C, sealed storage, away from moisture. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| H2O | 83.33 mg/mL (95.18 mM) | requires sonication |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture; avoid repeated freeze-thaw cycles.
If water is used as the stock solvent, dilute to the working solution and sterilize it through a 0.22 μm filter before use.
In Vivo
Choose the formulation that suits the animal model and route of administration. Percentages are volume ratios of the final working solution. Prepare the working solution fresh on the day of dosing; if precipitation or phase separation occurs, gentle warming or sonication can help.
Direct preparation of the working solution
These formulations are prepared directly, without a DMSO stock; use them promptly after preparation.
Protocol 1
| Composition | PBS |
|---|---|
| Result | 100 mg/mL (114.22 mM); clear solution; requires sonication |
Data provided by the manufacturer.
In Vitro
Acetyl coenzyme A trisodium raises cytoplasmic protein acetylation and lowers starvation-induced autophagic fluxes in starved U2OS cells (U2OS cells stably expressing GFP-LC3 are microinjected with Acetyl coenzyme A trisodium, incubated under nutrient-free conditions with 100 nM BafA1, and fixed after 3 h)[2].
In Vivo
In a mouse cardiac pressure overload model, Acetyl coenzyme A trisodium blunts pressure overload-induced cardiomyopathy by suppressing maladaptive autophagy[2][3]. In mice deprived of food for 24 h (with water available ad libitum), total Acetyl coenzyme A trisodium levels fall significantly in several organs, including the heart and muscles, matching a decrease in protein acetylation levels. Under the same conditions, however, Acetyl coenzyme A trisodium concentrations in the brain are not majorly affected, while hepatic Acetyl coenzyme A trisodium and protein acetylation levels actually rise[4].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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A Citrate Synthase Splice Variant Rewires the TCA Cycle to Promote Colorectal Cancer Progression. Cancer Res 2025 Sep 3. PMID: 40900036
Osteoclasts control endochondral ossification via regulating acetyl-CoA availability. Bone Res 2024 Aug 28;12(1):49. PMID: 39198395
mTOR-NAA10-C7orf50 axis senses nutritional status to coordinate ribosome biogenesis and autophagy. Sci Adv 2026 May 15;12(20):eadz3835. PMID: 42139339
Ergosterol alleviates hepatic steatosis and insulin resistance via promoting fatty acid β-oxidation by activating mitochondrial ACSL1. Cell Rep 2025 Jan 10;44(1):115203. PMID: 39799570
Chuangling Ye mitigates diabetic foot ulcer through suppressing keratinocyte ferroptosis via inhibiting ACSS2/ACSL4 axis. Chin Med 2026 Jun 8;21(1):162. PMID: 42260608
Identification of key events and regulatory networks in the formation process of primordial germ cell based on proteomics. J Cell Physiol 2023 Mar;238(3):610-630. PMID: 36745473
A liquid chromatography-mass spectrometry method to quantify total Coenzyme A concentration and isotopic labeling. bioRxiv 2026 May 20:2026.05.19.726225. PMID: 42239474
