AChR Alpha9 Polyclonal Antibody

SKU:BHA12103479
Overview
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CHRNA9-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, ELISA in Human, Mouse, Rat samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen The antiserum was produced against synthesized peptide derived from the N-terminal region of human CHRNA9. AA range:50-100
Molecular Weight ~54.8 kDa
UniProt Q9UGM1
Gene ID Human: 55584
Applications 2 validated
Available Options
Size
Options selector
Catalog no. Size
BT-AP00175-20UL 20 uL
BT-AP00175-50UL 50 uL
BT-AP00175-100UL 100 uL
Field Specification
Alternative Names Neuronal acetylcholine receptor subunit alpha-9 (Nicotinic acetylcholine receptor subunit alpha-9) (NACHR alpha-9)
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 55584
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from the N-terminal region of human CHRNA9. AA range:50-100
Isotype
  • IgG
Molecular Weight ~54.8 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Rat
Storage -20°C for 1 year
Target CHRNA9

Product Overview

Primary Antibody

This antibody targets CHRNA9 and detects endogenous levels of CHRNA9 protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target CHRNA9
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Molecular Weight ~54.8 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from the N-terminal region of human CHRNA9. AA range:50-100
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 55584
UniProt Q9UGM1 (Human: Q9UGM1; Rat: P43144)

Scientific Background

CHRNA9 (cholinergic receptor nicotinic alpha 9 subunit) is a member of the ligand-gated ionic channel family and nicotinic acetylcholine receptor gene superfamily. It encodes a plasma membrane protein that forms homo- or hetero-oligomeric divalent cation channels. This protein is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea.

Alternative Names

Neuronal acetylcholine receptor subunit alpha-9 (Nicotinic acetylcholine receptor subunit alpha-9) (NACHR alpha-9)

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetCHRNA9
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Molecular Weight~54.8 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from the N-terminal region of human CHRNA9. AA range:50-100
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 55584
UniProtQ9UGM1 (Human: Q9UGM1; Rat: P43144)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

CHRNA9 (cholinergic receptor nicotinic alpha 9 subunit) is a member of the ligand-gated ionic channel family and nicotinic acetylcholine receptor gene superfamily. It encodes a plasma membrane protein that forms homo- or hetero-oligomeric divalent cation channels. This protein is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea.

Alternative Names

Neuronal acetylcholine receptor subunit alpha-9 (Nicotinic acetylcholine receptor subunit alpha-9) (NACHR alpha-9)

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of CHRNA9?
The predicted molecular weight of CHRNA9 is ~54.8 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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