ACOT4 Polyclonal Antibody

SKU:BHA12101513
Overview
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ACOT4-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunohistochemistry, Immunofluorescence in Human samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
Molecular Weight ~46.3 kDa
UniProt Q8N9L9
Gene ID Human: 122970
Applications 4 validated
Options selector
Catalog no. Size
BT-AP00188-20UL 20 uL
BT-AP00188-50UL 50 uL
BT-AP00188-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-AP00188
Alternative Names ACOT4; PTE2B; PTEIB; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 122970
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410
Isotype
  • IgG
Molecular Weight ~46.3 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
Storage -20°C for 1 year
Target ACOT4

Product Overview

Primary Antibody

This antibody targets ACOT4 and detects endogenous levels of ACOT4 protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC) Refer to datasheet for recommended working dilution
Immunofluorescence (IF) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target ACOT4
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human
Purification Affinity Purification
Molecular Weight ~46.3 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 122970
UniProt Q8N9L9 (Human: Q8N9L9; Mouse: Q8BWN8)

Scientific Background

Acyl-coenzyme A thioesterase 4 is an enzyme encoded by the ACOT4 gene in humans and is mapped to chromosome 14.In peroxisomes, it helps in β-oxidation of dicarboxylic acids to form succinate. ACOT4 is also responsible for the termination of β-oxidation of dicarboxylic acids of medium-chain length with the concomitant release of the corresponding free acids.

Alternative Names

ACOT4; PTE2B; PTEIB; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetACOT4
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman
PurificationAffinity Purification
Molecular Weight~46.3 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 122970
UniProtQ8N9L9 (Human: Q8N9L9; Mouse: Q8BWN8)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC)Refer to datasheet for recommended working dilution
Immunofluorescence (IF)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

Acyl-coenzyme A thioesterase 4 is an enzyme encoded by the ACOT4 gene in humans and is mapped to chromosome 14.In peroxisomes, it helps in β-oxidation of dicarboxylic acids to form succinate. ACOT4 is also responsible for the termination of β-oxidation of dicarboxylic acids of medium-chain length with the concomitant release of the corresponding free acids.

Alternative Names

ACOT4; PTE2B; PTEIB; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib

What species does this antibody react with?
This antibody has been validated for reactivity against Human. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of ACOT4?
The predicted molecular weight of ACOT4 is ~46.3 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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