| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
ACVR1C, also known as ALK7, is a type I serine/threonine kinase receptor of the TGF-beta superfamily. Together with type II receptors, it binds ligands such as Nodal, Activin B, and GDF3, leading to phosphorylation of SMAD2 and SMAD3 and activation of downstream transcriptional responses. Through this pathway, ACVR1C regulates cell growth, differentiation, apoptosis, and metabolic processes, with notable roles in adipose tissue function, insulin sensitivity, and pancreatic and reproductive biology. Because TGF-beta superfamily signaling influences both normal development and disease, including metabolic disorders and cancer, ACVR1C is a useful target for studies of receptor-mediated SMAD signaling.
Product Description & Applications
The ACVR1C ORF cDNA Lentivirus delivers lentiviral particles packaged with an expression vector for human ACVR1C. A CMV or EF1a promoter drives the ORF cDNA fused to a C-terminal epitope tag, while a fluorescent reporter (GFP, RFP, or BFP) and drug-selection marker (puromycin or blasticidin) are separated by a self-cleaving peptide for independent expression from the same transcript; in some constructs the reporter and marker are driven separately by a PGK promoter. ORF cloning accuracy has been confirmed by sequencing and protein expression validated by transient transfection. The vectors provide high, stable ACVR1C expression and reliable genome integration, enabling long-term stable cell lines. They efficiently transduce difficult-to-transfect cells, including primary and thawed cells, for studies of TGF-beta superfamily and SMAD signaling.
About This Product
This ORF cDNA lentivirus enables stable overexpression of ACVR1C (NCBI Accession: NM_145259) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (BFP, GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
