| Field | Specification |
|---|---|
| Target | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide of human ADO |
| Conjugate | |
| Purification | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes ADO. It is validated for Western Blot, Immunohistochemistry.
Validated Applications
| Applications | Western Blot, Immunohistochemistry |
|---|---|
| WB | 1:1000-1:5000 |
| IHC | 1:25-1:100 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic peptide of human ADO
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ADO |
| Purification | Affinity Purification |
| Concentration | 0.3 mg/mL |
| Storage Buffer | PBS with 0.05% sodium azide and 50% glycerol, pH 7.4 |
| Calculated MW | 30kDa |
| UniProt # | Q96SZ5 |
| Lead Time | 2-3 business days |
| Validity | 12 months |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice packs |
| Target Synonyms | 2 aminoethanethiol dioxygenase;2-aminoethanethiol dioxygenase;ADO 2 aminoethanethiol (cysteamine) dioxygenase;ADO;AEDO;C10orf22;Chromosome 10 open reasing frame 22;cysteamine (2-aminoethanethiol) dioxygenase (ADO);Cysteamine dioxygenase;DKFZp564C046;FLJ14547 |
Reactivity Notes
Validated reactivity: Human, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for ADO research from Elabscience Biotechnology Co Ltd, or contact us for alternative formats.
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