| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human AER61 was used as the immunogen for the EOGT antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
AER61 Antibody / EOGT is a anti-AER61 Rabbit antibody Recombinant Rabbit Monoclonal clone 30E02 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: AER61
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30E02, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
EOGT antibody is widely used in studies of glycosylation, signaling, and developmental biology. By detecting EOGT, researchers can explore how O GlcNAc modification influences Notch signaling, growth factor binding, and tissue morphogenesis. Mutations in EOGT are associated with Adams Oliver syndrome, a developmental disorder characterized by limb and scalp defects, underscoring its biological importance.
In western blot assays, EOGT antibody detects protein bands corresponding to the enzyme in tissue and cell extracts. Immunohistochemistry maps expression in developing tissues, while immunofluorescence reveals subcellular localization consistent with endoplasmic reticulum and Golgi compartments. These methods support detailed analysis of glycosyltransferase biology.
EOGT mediated O GlcNAc modification contributes to signaling regulation in vascular and neural development. Dysregulation of this pathway has implications in congenital disorders, cancer, and immune regulation. By applying EOGT antibody, scientists can investigate how defects in protein glycosylation contribute to human disease and identify therapeutic strategies that target glycosylation pathways.
EOGT antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
