| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | Up to 25mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 5-(5-iodo-4-methoxy-2-propan-2-ylphenoxy)pyrimidine-2,4-diamine.
- Also known as: RO-4
- CAS number: 865305-30-2
- Molecular formula: C14H17IN4O2.
- Purity: >95% (HPLC)
- Concentration: 1 nM - 10 µM.
- Form: Lyophilized powder.
- Solubility: Up to 25mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: Up to 25mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: P2X3 receptors
- Source: Synthetic
- Activity: AF-353 is a highly potent and selective P2X3 and P2X2/3 receptor antagonist. pIC50 for these receptors, measured using intracellular calcium assays and whole-cell voltage-clamp, range from 7.4 to 8.51.
- Alternative names: RO-4
Scientific background
AF-353 is a highly potent and selective antagonist of P2X3 and P2X2/3 purinergic receptors. The compound is orally bioavailable and is being used as an in vivo tool for studying the role of P2X channels in animal pain models.P2X receptors are a family of ion channels that are gated by ATP. They are expressed in cell types of almost every origin, including neuronal, muscular, epithelial and immune and have been shown to play a pivotal role in models of various pain conditions1.Antagonism of P2X3 receptors can reduce arterial pressure and basal sympathetic activity and normalizes carotid body hyper-reflexia in rats with hypertension2.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
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- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
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