| Field | Specification |
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| Target | |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human CD154 protein |
| Conjugate | |
| Purity | |
| Purification | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation.
Product Overview
This monoclonal antibody (clone 5C8), raised in Mouse, recognizes CD154. It is validated for Flow Cytometry, Blocking.
Validated Applications
| Applications | Flow Cytometry, Blocking |
|---|
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Recombinant Human CD154 protein
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 5C8 |
| Isotype | Mouse IgG2a, κ |
| Conjugate | Unconjugated (AF/LE: azide-free, low endotoxin) |
| Species Reactivity | Human, Monkey |
| Immunogen | Recombinant Human CD154 protein |
| Purification | Protein A/G Purified |
| Purity | >98% |
| Concentration | ≥ 1 mg/mL |
| Storage Buffer | Sterile PBS, pH 7.2 < 1.0 EU per mg of the antibody as determined by the LAL method. |
| UniProt # | P29965 |
| Gene ID | 959 |
| Lead Time | 2-3 business days |
| Validity | 12 months |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice packs |
| Target Synonyms | T-BAM; TNFSF5; TRAP; gp39;CD40L |
Reactivity Notes
Validated reactivity: Human, Monkey. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for CD154 research from Elabscience Biotechnology Co Ltd, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
